Antimicrobial and Antibiofilm Effects of Human Amniotic/Chorionic Membrane Extract on Streptococcus pneumoniae

Frontiers in Microbiology
Mukesh K YadavJae-Jun Song

Abstract

Background:Streptococcus pneumoniae colonize the human nasopharynx in the form of biofilms. The biofilms act as bacterial reservoirs and planktonic bacteria from these biofilms can migrate to other sterile anatomical sites to cause pneumonia, otitis media (OM), bacteremia and meningitis. Human amniotic membrane contains numerous growth factors and antimicrobial activity; however, these have not been studied in detail. In this study, we prepared amniotic membrane extract and chorionic membrane extract (AME/CME) and evaluated their antibacterial and antibiofilm activities against S. pneumoniae using an in vitro biofilm model and in vivo OM rat model. Materials and Methods: The AME/CME were prepared and protein was quantified using DC(TM) (detergent compatible) method. The minimum inhibitory concentrations were determined using broth dilution method, and the synergistic effect of AME/CME with Penicillin-streptomycin was detected checkerboard. The in vitro biofilm and in vivo colonization of S. pneumoniae were studied using microtiter plate assay and OM rat model, respectively. The AME/CME-treated biofilms were examined using scanning electron microscope and confocal microscopy. To examine the constituents of AME/CME, we determined...Continue Reading

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Citations

Jan 11, 2018·Journal of Functional Biomaterials·Yong MaoJoachim Kohn
Sep 11, 2019·Critical Reviews in Microbiology·Maria Daniela Silva, Sanna Sillankorva
Dec 24, 2018·Current Eye Research·Rehab Rashad Kassem, Randa Mohamed Abdel-Moneim El-Mofty
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Apr 9, 2020·Frontiers in Microbiology·Taja Železnik RamutaMateja Erdani Kreft
Feb 2, 2021·Frontiers in Bioengineering and Biotechnology·Fatemeh Dadkhah TehraniAzadeh Shabani
Dec 24, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Sarah GulameabasseMathilde Fenelon
Jul 13, 2021·Frontiers in Bioengineering and Biotechnology·Aleksandar JanevMateja Erdani Kreft
Jun 18, 2021·Frontiers in Bioengineering and Biotechnology·Taja Železnik RamutaMateja Erdani Kreft
Aug 18, 2021·Technology in Cancer Research & Treatment·Ameneh JafariHakimeh Zali
Aug 17, 2021·Frontiers in Bioengineering and Biotechnology·Palaniappan RamasamySekar Vijayakumar
Dec 9, 2020··Ernie Maduratna SetyawatiOkkinardo Arief

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Methods Mentioned

BETA
biopsies
lavage
Protein Assay
confocal microscopy

Software Mentioned

MASCOT
SPSS Statistics package
SPSS

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