Antimicrobial and Chemotactic Activity of Scorpion-Derived Peptide, ToAP2, against Mycobacterium massiliensis

Toxins
Lázaro M Marques-NetoAna Paula Junqueira-Kipnis

Abstract

Mycobacterium massiliense is a rapid growing, multidrug-resistant, non-tuberculous mycobacteria that is responsible for a wide spectrum of skin and soft tissue infections, as well as other organs, such as the lungs. Antimicrobial peptides had been described as broad-spectrum antimicrobial, chemotactic, and immunomodulator molecules. In this study we evaluated an antimicrobial peptide derived from scorpion Tityus obscurus as an anti-mycobacterial agent in vitro and in vivo. Bioinformatics analyses demonstrated that the peptide ToAP2 have a conserved region similar to several membrane proteins, as well as mouse cathelicidin. ToAP2 inhibited the growth of four M. massiliense strains (GO01, GO06, GO08, and CRM0020) at a minimal bactericidal concentration (MBC) of 200 µM. MBC concentration used to treat infected macrophages was able to inhibit 50% of the bacterial growth of all strains. ToAP2 treatment of infected mice with bacilli reduced the bacterial load in the liver, lung, and spleen, similarly to clarithromycin levels (90%). ToAP2 alone recruited monocytes (F4/80low Gr1), neutrophils (F4/80- Gr1), and eosinophils (F4/80+ Gr1+). ToAP2, together with M. massiliense infection, was able to increase F4/80low and reduce the percenta...Continue Reading

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Citations

Jan 18, 2020·Antibiotics·Clara M BentoTânia Silva
Jul 21, 2020·Archiv der Pharmazie·Elida C G da MataElisabeth F Schwartz
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Mar 4, 2020·Toxicon : Official Journal of the International Society on Toxinology·Laura L Valdez-VelázquezLourival D Possani

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Methods Mentioned

BETA
circular dichroism
solid phase chromatography
lavage
flow cytometry

Software Mentioned

GraphPad
LALIGN
iTasser
Clustal Omega
Flowjo Vx
Prism
FlexAnalysis
FlowJO
PSIPRED
Excel

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