Antimicrobial biosynthetic potential and diversity of culturable soil actinobacteria from forest ecosystems of Northeast India.

Scientific Reports
Priyanka Sharma, Debajit Thakur

Abstract

Actinobacteria is a goldmine for the discovery of abundant secondary metabolites with diverse biological activities. This study explores antimicrobial biosynthetic potential and diversity of actinobacteria from Pobitora Wildlife Sanctuary and Kaziranga National Park of Assam, India, lying in the Indo-Burma mega-biodiversity hotspot. A total of 107 actinobacteria were isolated, of which 77 exhibited significant antagonistic activity. 24 isolates tested positive for at least one of the polyketide synthase type I, polyketide synthase type II or non-ribosomal peptide synthase genes within their genome. Their secondary metabolite pathway products were predicted to be involved in the production of ansamycin, benzoisochromanequinone, streptogramin using DoBISCUIT database. Molecular identification indicated that these actinobacteria predominantly belonged to genus Streptomyces, followed by Nocardia and Kribbella. 4 strains, viz. Streptomyces sp. PB-79 (GenBank accession no. KU901725; 1313 bp), Streptomyces sp. Kz-28 (GenBank accession no. KY000534; 1378 bp), Streptomyces sp. Kz-32 (GenBank accession no. KY000536; 1377 bp) and Streptomyces sp. Kz-67 (GenBank accession no. KY000540; 1383 bp) showed ~89.5% similarity to the nearest type ...Continue Reading

References

Jan 1, 1991·Journal of Bacteriology·W G WeisburgD J Lane
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Apr 13, 1999·Journal of Applied Microbiology·M L Niku-PaavolaA Haikara
Mar 8, 2000·Nature·N MyersJ Kent
Jun 1, 2000·Nature·H OchmanE A Groisman
Jun 23, 2001·The Journal of Antimicrobial Chemotherapy·J M Andrews
Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·S OmuraM Hattori
Apr 15, 2003·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M F VicenteF Peláez
Dec 6, 2003·International Journal of Systematic and Evolutionary Microbiology·Andrew E Cook, Paul R Meyers
Feb 28, 2004·Journal of Natural Products·Gary StrobelJames Harper
Oct 30, 2004·Chembiochem : a European Journal of Chemical Biology·Andriy LuzhetskyyAndreas Bechthold
Apr 9, 2005·The Journal of Antibiotics·János Bérdy
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J V McArthurM H Smith
May 23, 2006·Journal of Biotechnology·Giorgos SianidisAnastassios Economou
Dec 16, 2006·Journal of Industrial Microbiology & Biotechnology·Wei-Hua Chu
Apr 5, 2007·Chembiochem : a European Journal of Chemical Biology·Andreas HornungStefan Pelzer
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Jul 13, 2007·Journal of the American Chemical Society·Gerald LacknerChristian Hertweck
Oct 4, 2007·International Journal of Systematic and Evolutionary Microbiology·Jongsik ChunYoung-Woon Lim
Sep 24, 2008·Current Microbiology·Ramesh Chand KasanaArvind Gulati
Feb 16, 2010·Applied and Environmental Microbiology·Erin A GontangPaul R Jensen

❮ Previous
Next ❯

Datasets Mentioned

BETA
KY271082
KM244742
KU892679
KY000533

Methods Mentioned

BETA
light microscopy
PCR
electrophoresis

Software Mentioned

EzTaxon
SimQual
DoBISCUIT
VENNTURE
MEGA
ORF FINDER
MEGA ( Molecular Evolutionary Genetics Analysis )
BLAST
SPSS
NTSYS

Related Concepts

Related Feeds

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.