Antimicrobial mechanism of action of transferrins: selective inhibition of H+-ATPase.

Antimicrobial Agents and Chemotherapy
María T Andrés, José F Fierro

Abstract

Two bacterial species with different metabolic features, namely, Pseudomonas aeruginosa and Lactococcus lactis, were used as a comparative experimental model to investigate the antimicrobial target and mechanism of transferrins. In anaerobiosis, P. aeruginosa cells were not susceptible to lactoferrin (hLf) or transferrin (hTf). In aerobiosis, the cells were susceptible but O(2) consumption was not modified, indicating that components of the electron transport chain (ETC) were not targeted. However, the respiratory chain inhibitor piericidin A significantly reduced the killing activity of both proteins. Moreover, 2,6-dichlorophenolindophenol (DCIP), a reducing agent that accepts electrons from the ETC coupled to H(+) extrusion, made P. aeruginosa susceptible to hLf and hTf in anaerobiosis. These results indicated that active cooperation of the cell was indispensable for the antimicrobial effect. For L. lactis cells lacking an ETC, the absence of a detectable transmembrane electrical potential in hLf-treated cells suggested a loss of H(+)-ATPase activity. Furthermore, the inhibition of ATPase activity and H(+) translocation (inverted membrane vesicles) provided direct evidence of the ability of hLf to inhibit H(+)-ATPase in L. la...Continue Reading

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Citations

Jul 31, 2013·Journal of Agricultural and Food Chemistry·Dilip K LakshmanAmitava Mitra
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Michaela WenzelJulia Elisabeth Bandow
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Jan 7, 2021·International Journal of Nanomedicine·Reham Samir HamidaMayasar Ibrahim Al-Zaban
Sep 25, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Seema Patel, Nadeem Akhtar
Jul 9, 2021·International Journal of Biological Macromolecules·Cátia Santos-PereiraSérgio F Sousa
Jul 6, 2021·Toxicology Mechanisms and Methods·Mohammad Rafiq Wani, G G Hammad Ahmad Shadab
Apr 28, 2018·Microbial Pathogenesis·N G VasconcelosS Simionatto
Mar 12, 2020·ACS Infectious Diseases·Declan Alan Gray, Michaela Wenzel

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