Antimicrobial peptaibols, novel suppressors of tumor cells, targeted calcium-mediated apoptosis and autophagy in human hepatocellular carcinoma cells.

Molecular Cancer
Mei ShiYu-Zhong Zhang

Abstract

Hepatocellular carcinoma (HCC) is one of the most common cancers in the world which is highly chemoresistant to currently available chemotherapeutic agents. Thus, novel therapeutic targets are needed to be sought for the successful treatment of HCC. Peptaibols, a family of peptides synthesized non-ribosomally by the Trichoderma species and other fungi, exhibit antibiotic activities against bacteria and fungi. Few studies recently showed that peptaibols exerted cytotoxicity toward human lung epithelial and breast carcinoma cells. However, the mechanism involved in peptaibol-induced cell death remains poorly understood. Here, we showed that Trichokonin VI (TK VI), a peptaibol from Trichoderma pseudokoningii SMF2, induced growth inhibition of HCC cells in a dose-dependent manner. It did not obviously impair the viability of normal liver cells at lower concentration. Moreover, the suppression of cell viability resulted from the programmed cell death (PCD) with characteristics of apoptosis and autophagy. An influx of Ca2+ triggered the activation of mu-calpain and proceeded to the translocation of Bax to mitochondria and subsequent promotion of apoptosis. On the other hand, typically morphological characteristics consistent with aut...Continue Reading

References

Jul 20, 1977·Journal of the American Chemical Society·R C PandeyK L Rinehart
Dec 12, 1994·European Journal of Pharmacology·Q HuangY Momose
Oct 1, 1995·Chemical & Pharmaceutical Bulletin·Q HuangK Tubaki
May 2, 2000·Applied and Environmental Microbiology·M R HermosaI Garcia-Acha
Aug 11, 2001·Biochemical Society Transactions·J K Chugh, B A Wallace
Oct 2, 2001·Cellular and Molecular Life Sciences : CMLS·C TonioloR M Epand
Nov 1, 2001·The Journal of Membrane Biology·H Duclohier, H Wróblewski
Mar 23, 2002·The Journal of Biological Chemistry·Aric WiestCharles Kenerley
Jun 20, 2002·The Journal of Biological Chemistry·Ida Stenfeldt MathiasenMarja Jäättelä
Nov 25, 2003·Oncogene·Rosario RizzutoTullio Pozzan
Dec 4, 2003·Current Opinion in Cell Biology·Tomomi Kuwana, Donald D Newmeyer
Dec 9, 2003·Journal of Peptide Science : an Official Publication of the European Peptide Society·Kathrin ReiberTorsten Schwecke
Jun 23, 2004·The Journal of Biological Chemistry·Ashish K Sharma, Baerbel Rohrer
Aug 6, 2004·Applied and Environmental Microbiology·Joanna PeltolaMirja S Salkinoja-Salonen
Sep 4, 2004·Biophysical Journal·H DuclohierB A Wallace
Jan 28, 2005·Journal of Cell Science·Safa Lucken-Ardjomande, Jean-Claude Martinou
Oct 6, 2005·International Journal of Cancer. Journal International Du Cancer·Ching Tung LumHsiang Fu Kung
Feb 4, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Esther BuytaertPatrizia Agostinis
Oct 4, 2006·Molecular and Cellular Biology·Philippe A ParoneJean-Claude Martinou
Jan 16, 2007·Apoptosis : an International Journal on Programmed Cell Death·Surajit KarmakarSwapan K Ray
Feb 6, 2007·Developmental Cell·Sarah Swerdlow, Clark W Distelhorst
Oct 26, 2007·International Journal of Cancer. Journal International Du Cancer·Che-Hsin LeeAi-Li Shiau
Nov 9, 2007·Apoptosis : an International Journal on Programmed Cell Death·Andrew Thorburn
Dec 14, 2007·Trends in Cell Biology·Claudio Hetz, Laurie Glimcher
Dec 18, 2007·Cell·David E Clapham
Mar 1, 2008·Cell Death and Differentiation·L GalluzziG Kroemer
Jul 17, 2008·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Keith Singletary, John Milner
Jul 24, 2008·Chemistry & Biodiversity·Subrayashastry AravindaPadmanabhan Balaram
Dec 17, 2008·Cell Death and Differentiation·N Kourtis, N Tavernarakis
Jan 14, 2009·Autophagy·Qinghua CuiElizabeth Yang
Feb 24, 2009·Trends in Molecular Medicine·Ya-Ching HsiehIrshad H Chaudry
Oct 24, 2012·Autophagy·Paul G ThomesTerrence M Donohue

❮ Previous
Next ❯

Citations

Dec 18, 2012·Apoptosis : an International Journal on Programmed Cell Death·Mei ShiYu-Zhong Zhang
Dec 3, 2010·The Journal of Biological Chemistry·Prasun K MukherjeeCharles M Kenerley
Jun 2, 2011·Journal of Biomedicine & Biotechnology·Hidemi Rikiishi
Feb 8, 2014·International Journal of Molecular Sciences·Azhar NajjarYoussuf Gherbawy
Dec 17, 2014·The Journal of Chemical Physics·Michael A WilsonAndrew Pohorille
Dec 22, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Anne-Isaline Van BohemenYves François Pouchus
Oct 2, 2015·Acta Biochimica Et Biophysica Sinica·Xiaodong CuiZhuanhua Wang
Jun 30, 2012·Journal of Peptide Science : an Official Publication of the European Peptide Society·Sloan AyersNicholas H Oberlies
Mar 20, 2012·Biochimica Et Biophysica Acta·Edgar J Paredes-GameroAntonio Miranda
Dec 18, 2016·Journal of Cellular Biochemistry·Marcus V BuriEdgar J Paredes-Gamero
Apr 11, 2018·Archives of Insect Biochemistry and Physiology·Marziyeh BadinlooSvetlana N Radyuk
Apr 18, 2018·Journal of Experimental & Clinical Cancer Research : CR·Ling CaoYi Zhang
Oct 27, 2017·Proceedings of the National Academy of Sciences of the United States of America·Lin DuRobert H Cichewicz
May 18, 2013·Chemistry & Biodiversity·Angélique CarrouxNicolas Ruiz
Oct 31, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Eugene A RogozhinYaroslav A Andreev
Apr 11, 2020·International Journal of Molecular Sciences·Piotr SiupkaZofia Piotrowska-Seget
Nov 20, 2012·Chemistry & Biodiversity·Nicolas PradeilleHeinz Heimgartner
May 18, 2013·Chemistry & Biodiversity·Pia BlaserHeinz Heimgartner
Feb 19, 2021·Journal of Natural Products·Anne-Isaline van BohemenYves François Pouchus

❮ Previous
Next ❯

Datasets Mentioned

BETA
FJ605099

Methods Mentioned

BETA
fluorescence microscopy
FACS
electrophoresis
DNA
flow cytometry
transmission electron microscopy
transfection
protein assay
PCR

Software Mentioned

SPSS
CellQuest
FlowJo

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Autophagy & Disease

Autophagy is an important cellular process for normal physiology and both elevated and decreased levels of autophagy are associated with disease. Here is the latest research.

Autophagy & Metabolism

Autophagy preserves the health of cells and tissues by replacing outdated and damaged cellular components with fresh ones. In starvation, it provides an internal source of nutrients for energy generation and, thus, survival. A powerful promoter of metabolic homeostasis at both the cellular and whole-animal level, autophagy prevents degenerative diseases. It does have a downside, however--cancer cells exploit it to survive in nutrient-poor tumors.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Parkinson's Disease & Autophagy (MDS)

Autophagy leads to degradation of damaged proteins and organelles by the lysosome. Impaired autophagy has been implicated in several diseases. Here is the role of autophagy in Parkinson’s disease.

Adrenocortical Carcinoma

Hepatocellular carcinoma is the most common type of primary liver cancer and frequently occurs in individuals with chronic liver diseases like cirrhosis. Here is the latest research.

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms