Antimicrobial peptide magainin I from Xenopus skin forms anion-permeable channels in planar lipid bilayers.

Biophysical Journal
H DuclohierG Spach

Abstract

The ionophore properties of magainin I, an antimicrobial and amphipathic peptide from the skin of Xenopus, were investigated in planar lipid bilayers. Circular dichroism studies, performed comparatively with alamethicin, in small or large unilamellar phospholipidic vesicles, point to a smaller proportion of alpha-helical conformation in membranes. A weakly voltage-dependent macroscopic conductance which is anion-selective is developed when using large aqueous peptide concentration with lipid bilayer under high voltages. Single-channel experiments revealed two main conductance levels occurring independently in separate trials. Pre-aggregates lying on the membrane surface at rest and drawn into the bilayer upon voltage application are assumed to account for this behaviour contrasting with the classical multistates displayed by alamethicin.

Citations

Oct 12, 1999·Biopolymers·B BechingerS Schinzel
Dec 20, 2002·Biopolymers·Yechiel Shai
Dec 1, 1993·Protein Science : a Publication of the Protein Society·B BechingerS J Opella
Oct 1, 1994·Protein Science : a Publication of the Protein Society·M PawlakH Vogel
May 1, 1996·Journal of Peptide Science : an Official Publication of the European Peptide Society·S V SychevV T Ivanov
Dec 1, 2007·Journal of Biological Physics·Henry D Herce, Angel E Garcia
Jan 1, 1991·The International Journal of Biochemistry·T Cserháti, M Szögyi
Jan 1, 1992·Advances in Enzyme Regulation·J H Spencer
Feb 1, 1989·Journal of the American College of Cardiology·D R Hathaway, K L March
Aug 3, 1992·European Journal of Pharmacology·R A CrucianiE F Stanley
Feb 1, 1992·Current Opinion in Immunology·M Zasloff
Dec 4, 2003·Biochimica Et Biophysica Acta·Yoshiro NakajimaMinoru Yamakawa
Feb 11, 2000·Toxicon : Official Journal of the International Society on Toxinology·S HaeberliW Nentwig
May 10, 2000·Journal of Microbiological Methods·G Mardones, A Venegas
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·R A CrucianiO Colamonici
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·G DiamondC L Bevins
Jan 29, 2005·The Journal of Biological Chemistry·Yuxin ChenRobert S Hodges
Aug 1, 1994·The Journal of Applied Bacteriology·E M JonesM R Millar
Oct 1, 1991·European Journal of Biochemistry·H G BomanD A Lidholm
Sep 15, 1994·European Journal of Biochemistry·V CabiauxJ M Ruysschaert
Feb 1, 1991·Antimicrobial Agents and Chemotherapy·M J ArrowoodM C Healey
Mar 1, 1995·European Journal of Biochemistry·H V WesterhoffD Juretić
Jan 15, 1994·European Journal of Biochemistry·A Mor, P Nicolas
Mar 14, 2001·Journal of Peptide Science : an Official Publication of the European Peptide Society·E DéF Heitz
Nov 29, 2015·Colloids and Surfaces. B, Biointerfaces·Jessie PeyreClaire-Marie Pradier
Jan 22, 2008·Toxicon : Official Journal of the International Society on Toxinology·Manoel Arcisio-MirandaJoaquim Procopio
Mar 28, 2013·Scientific Reports·Michelle L GeeAndrew H A Clayton
Jul 11, 2009·Journal of Peptide Science : an Official Publication of the European Peptide Society·Ryan W DavisMichael B Sinclair
Feb 24, 2006·Chemical Biology & Drug Design·Yuxin ChenRobert S Hodges
Aug 2, 2011·Journal of Peptide Science : an Official Publication of the European Peptide Society·Luiz C SalayValtencir Zucolotto

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.