Antimicrobial peptide protegrin-3 adopt an antiparallel dimer in the presence of DPC micelles: a high-resolution NMR study

Journal of Biomolecular NMR
K S UsachevV V Klochkov

Abstract

A tendency to dimerize in the presence of lipids was found for the protegrin. The dimer formation by the protegrin-1 (PG-1) is the first step for further oligomeric membrane pore formation. Generally there are two distinct model of PG-1 dimerization in either a parallel or antiparallel β-sheet. But despite the wealth of data available today, protegrin dimer structure and pore formation is still not completely understood. In order to investigate a more detailed dimerization process of PG-1 and if it will be the same for another type of protegrins, in this work we used a high-resolution NMR spectroscopy for structure determination of protegrin-3 (RGGGL-CYCRR-RFCVC-VGR) in the presence of perdeuterated DPC micelles and demonstrate that PG-3 forms an antiparallel NCCN dimer with a possible association of these dimers. This structural study complements previously published solution, solid state and computational studies of PG-1 in various environments and validate the potential of mean force simulations of PG-1 dimers and association of dimers to form octameric or decameric β-barrels.

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Citations

Apr 7, 2017·Journal of Peptide Science : an Official Publication of the European Peptide Society·Richard Lipkin, Themis Lazaridis
Apr 21, 2019·Medicinal Research Reviews·Thanh-Nhat PhamPascal Sonnet
Jun 21, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Richard Lipkin, Themis Lazaridis
Aug 10, 2019·Critical Reviews in Clinical Laboratory Sciences·Adriana Barreto-SantamaríaHernando Curtidor
Jun 3, 2018·European Biophysics Journal : EBJ·S V EfimovV V Klochkov
Aug 15, 2018·European Biophysics Journal : EBJ·Ruslan GarifullinTimur I Abdullin
Jun 24, 2018·Pharmaceutics·Ilaria PassariniMire Zloh
Jun 6, 2020·The Journal of Membrane Biology·Mykola V RodninThemis Lazaridis
Feb 11, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Galina M MamardashviliNugzar Zh Mamardashvili
Jun 8, 2021·Biochimica Et Biophysica Acta. Biomembranes·Beatriz LimaRicardo D Enriz
Sep 8, 2017·The Journal of Physical Chemistry. B·Richard LipkinThemis Lazaridis
May 29, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Baixiong ZhangShuji Ye

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