Antimicrobial peptides from diverse families isolated from the skin of the Asian frog, Rana grahami

Peptides
J Michael ConlonHubert Vaudry

Abstract

Seven peptides with antimicrobial activity were isolated in pure form from an extract of the skin of the Yunnanfu Kunming frog Rana grahami Boulenger, 1917. The peptides were identified as belonging to the nigrocin-2 (three peptides), brevinin-1 (one peptide), brevinin-2 (three peptides), and esculentin-1 (one peptide) families. Nigrocin-2GRb (GLFGKILGVGKKVLCGLSGMC) containing three lysine residues, represented the peptide with highest potency against microorganisms (MIC = 3 microM against Escherichia coli, 12.5 microM against Staphylococcus aureus and 50 microM against Candida albicans) and the greatest hemolytic activity against human erythrocytes (LD50 = 40 microM). In contrast, nigrocin-2GRa (GLLSGILGAGKHIVCGLSGLC) and nigrocin-2GRc (GLLSGILGAGKNIVCGLSGLC), with only a single lysine residue, showed weak antimicrobial and hemolytic activity. Phylogenetic relationships among Eurasian ranid frogs are less well understood than those of North American ranids but the primary structures of the R. grahami antimicrobial peptides suggest a close relationship of this species with the Japanese pond frogs R. nigromaculata and R. porosa brevipoda.

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Citations

Jan 5, 2014·PloS One·Ping MengRen Lai
Jul 16, 2008·Toxicon : Official Journal of the International Society on Toxinology·J Michael ConlonJay D King
Sep 22, 2007·Toxicon : Official Journal of the International Society on Toxinology·Anna NascimentoMariana S Castro
Mar 18, 2011·Journal of Peptide Science : an Official Publication of the European Peptide Society·V ReshmyS George
Aug 25, 2006·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·J Michael ConlonPer F Nielsen
Feb 11, 2015·Biomacromolecules·Prashant KumarSuzana K Straus
Feb 3, 2007·Molecular & Cellular Proteomics : MCP·Jianxu LiJing Wu
Jul 17, 2018·Current Medicinal Chemistry·Jiri PatockaKamil Kuca
Jan 1, 2015·Antibiotics·Vineeth T V KumarSanil George

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