Antimicrobial photodynamic inactivation of Staphylococcus aureus biofilms in bone specimens using methylene blue, toluidine blue ortho and malachite green: An in vitro study

Archives of Oral Biology
Luciano Pereira RosaMagda Souza Viana

Abstract

To evaluate the in vitro effectiveness of APDI with a 660 nm laser combined with methylene blue (MB), toluidine blue ortho (TBO) and malachite green (MG) dyes to inactivate Staphylococcus aureus (ATCC 25923) biofilms in compact and cancellous bone specimens. Eighty specimens of compact and 80 of cancellous bone were contaminated with a standard suspension of the microorganism and incubated for 14 days at 37°C to form biofilms. After this period, the specimens were divided into groups (n=10) according to established treatment: PS-L- (control - no treatment); PSmb+L-, PStbo+L-, PSmg+L- (only MB, TBO or MG for 5 min in the dark); PS-L+ (only laser irradiation for 180 s); and APDImb, APDItbo and APDImg (APDI with MB, TBO or MG for 180 s). The findings were statistically analyzed by ANOVA at 5% significance levels. All experimental treatments showed significant reduction of log CFU/mL S. aureus biofilms when compared with the control group for compact and cancellous bones specimens; the APDI group's treatment was more effective. The APDI carried out for the compact specimens showed better results when compared with cancellous specimens at all times of application. For the group of compact bone, APDImg showed greater reductions in CF...Continue Reading

References

Oct 10, 1998·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·R J BakemanR F Newhouse
Jan 23, 2003·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Conor P Barry, C David Ryan
May 4, 2004·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Michael R Hamblin, Tayyaba Hasan
Jan 6, 2006·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Stuart K BislandShane Burch
Mar 29, 2006·Journal of Environmental Pathology, Toxicology and Oncology : Official Organ of the International Society for Environmental Toxicology and Cancer·Giulio Jori
Jun 1, 2006·The Journal of Antimicrobial Chemotherapy·Daniel MetcalfSimon Wood
Sep 19, 2006·Journal of Photochemistry and Photobiology. B, Biology·Renato Araújo PratesMartha Simões Ribeiro
Jun 15, 2007·Journal of Photochemistry and Photobiology. B, Biology·Egberto MuninMaricilia Silva Costa
May 14, 2008·Antimicrobial Agents and Chemotherapy·George P TegosMichael R Hamblin
May 17, 2008·Experimental and Molecular Pathology·Travis J WilesMatthew A Mulvey
May 26, 2009·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Aysegul Mine Tuzuner-OnculReha S Kisnisci
Sep 18, 2009·European Journal of Oral Sciences·Juliana P M LimaIriana C J Zanin
Jan 16, 2010·Lasers in Surgery and Medicine·Tianhong DaiMichael R Hamblin
Nov 12, 2010·Lasers in Medical Science·Cristiane Aparecida PereiraAntonio Olavo Cardoso Jorge
Mar 2, 2011·Brazilian Oral Research·Michelle MiyabeIlíria Salomão Feist
Jan 3, 2012·Archives of Oral Biology·Simone Furgeri Godinho VilelaAntonio Olavo Cardoso Jorge
Mar 1, 2012·Photodiagnosis and Photodynamic Therapy·Nasim KashefGholamreza Esmaeeli Djavid

❮ Previous
Next ❯

Citations

Oct 10, 2015·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Sigurd HafnerAndreas Wieser
Apr 20, 2016·Journal of Photochemistry and Photobiology. B, Biology·Mark WainwrightSophie Rawthore
Aug 30, 2016·International Journal of Pharmaceutics·M S GualdesiC S Ortiz
Jun 27, 2017·Journal of Photochemistry and Photobiology. B, Biology·Hui LiangQingyong Li
Jun 25, 2017·Lasers in Medical Science·Palloma Porto AlmeidaRobson Amaro Augusto da Silva
Jun 16, 2018·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Mark Wainwright
Mar 23, 2019·Future Medicinal Chemistry·Joanna NakoniecznaMariusz Grinholc
Nov 22, 2018·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Esmaeil DarabpourAbbas Ali Imani Fooladi
Jun 15, 2017·Lasers in Medical Science·Lautaro GándaraAdriana Casas
Jul 13, 2018·Frontiers in Microbiology·Xiaoqing HuMichael R Hamblin
Feb 23, 2020·Photodiagnosis and Photodynamic Therapy·G A M D FonsecaL Frigo
Jan 7, 2020·Photodiagnosis and Photodynamic Therapy·Kanako ShitomiHideya Kawasaki
Apr 10, 2021·Photodiagnosis and Photodynamic Therapy·Camila PérezChristian Erick Palavecino
Jun 17, 2021·Photodiagnosis and Photodynamic Therapy·Samane ShahmoradiDavood Darban-Sarokhalil

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.

Actinomycosis

Actinomycosis is a rare, chronic, and slowly progressive granulomatous disease caused by filamentous Gram positive anaerobic bacteria from the Actinomycetaceae family (genus Actinomyces). The disease is characterised by the formation of painful abscesses in the mouth, lungs,breast or gastrointestinal tract. Discover the latest research on actinomycosis here.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.