Antimicrobial potential of Alpinia purpurata lectin (ApuL): Growth inhibitory action, synergistic effects in combination with antibiotics, and antibiofilm activity

Microbial Pathogenesis
Gustavo Ramos Salles FerreiraT H Napoleão

Abstract

The Alpinia purpurata inflorescence contains a lectin (ApuL), which has immunomodulatory activities on human cells. In the present work, it was evaluated the antibacterial and antifungal effects of ApuL against human pathogens. ApuL showed bacteriostatic activity against non-resistant (UFPEDA-02) and an oxacillin-resistant isolate (UFPEDA-672) of Staphylococcus aureus with minimal inhibitory concentrations (MIC50) of 50 and 400 μg/mL, respectively. In addition, it showed bactericidal effect on the non-resistant isolate (minimal bactericidal concentration: 200 μg/mL). For Candida albicans and Candida parapsilosis, ApuL showed fungistatic effect (MIC50: 200 and 400 μg/mL, respectively). The lectin was able to impair the viability of the microorganism cells, as indicated by propidium iodide (PI) staining. Analysis of growth curves, protein leakage, and ultrastructural changes supported that ApuL acts through distinct mechanisms on S. aureus isolates. Ultrastructural analysis of ApuL-treated Candida cells revealed malformations with elongations and bulges. ApuL-oxacillin combination showed synergistic effect on the oxacillin-resistant isolates UFPEDA-670 and 671, which were not sensitive to lectin alone. Synergism was also detected...Continue Reading

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Citations

Sep 7, 2019·Current Protein & Peptide Science·Marianela Del RioMariana Regente
Dec 5, 2019·Scientific Reports·Isana Maria de Souza Feitosa LimaLuís Cláudio Nascimento da Silva
Aug 11, 2019·Clinica Chimica Acta; International Journal of Clinical Chemistry·Priscila Marcelino Dos Santos SilvaMaria Tereza Dos Santos Correia
Nov 15, 2019·International Journal of Biological Macromolecules·Valdenice F SantosClaudener S Teixeira
Nov 27, 2020·Frontiers in Genetics·Madangchanok ImchenVasco Azevedo
Sep 21, 2020·Microbial Pathogenesis·Raí Emanuel da SilvaMaria José Dos Santos Soares
Jun 3, 2021·Antibiotics·João Victor de Oliveira SantosIsabella Macário Ferro Cavalcanti
Jun 16, 2021·Carbohydrate Research·Dixita ChettriAnil Kumar Verma
Jul 25, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·F J Álvarez-MartínezV Micol
Jun 7, 2019·International Journal of Biological Macromolecules·Pollyanna Michelle da SilvaThiago Henrique Napoleão
Aug 23, 2021·Probiotics and Antimicrobial Proteins·Suéllen Pedrosa da SilvaThiago Henrique Napoleão

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