Antimicrobial Potential of Single Metabolites of Curcuma longa Assessed in the Total Extract by Thin-Layer Chromatography-Based Bioautography and Image Analysis

International Journal of Molecular Sciences
Lidia CzernickaWirginia Kukula-Koch

Abstract

Curcuma longa from Zingiberaceae belongs to the major spices consumed around the world, known from its cholagogue, anti-inflammatory, and antimicrobial properties. Lack of data on the activity of single components of turmeric extract encouraged the authors to apply TLC (thin-layer chromatography) based bioautography studies to reveal its antimicrobial constituents and construct a universal platform for the bioactivity assessment of crude extracts, with help of a freeware ImageJ software. This optimized chromatographic bioassay performed on diethyl ether and methanol extracts of Curcuma longa was successfully applied on the total extract and revealed the antimicrobial potential of single components against a variety of Gram-positive strains, with no need for their isolation from the mixture. The obtained results were further confronted with a classic microdilution antimicrobial assay on the isolates, purified from the crude extracts by centrifugal partition chromatography in the following solvent system: heptane-chloroform-methanol-water (5:6:3:2) (v/v/v/v).

References

Jul 20, 1995·Cancer Letters·A J RubyR Kuttan
Dec 15, 1995·Journal of Ethnopharmacology·A ApisariyakulD Buddhasukh
Dec 10, 1999·Journal of Agricultural and Food Chemistry·P S NegiK K Sakariah
Jun 23, 2001·The Journal of Antimicrobial Chemotherapy·J M Andrews
Jun 13, 2002·Journal of Agricultural and Food Chemistry·Guddadarangavvanahally K JayaprakashaKunnumpurath K Sakariah
Nov 15, 2007·Molecular Pharmaceutics·Preetha AnandBharat B Aggarwal
Feb 12, 2013·Acta Odontologica Scandinavica·Prasanna NeelakantanJames L Gutmann
May 31, 2014·BioMed Research International·Soheil Zorofchian MoghadamtousiKeivan Zandi
Apr 2, 2015·Journal of Food Science and Technology·Parveen Gul, Jehan Bakht
Apr 19, 2017·Journal of Traditional and Complementary Medicine·Augustine AmalrajSreeraj Gopi
May 22, 2017·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Krystyna Skalicka-WoźniakHosam O Elansary
Sep 5, 2017·Journal of Ayurveda and Integrative Medicine·Pooja D Gupta, Tannaz J Birdi
Sep 9, 2017·Phytotherapy Research : PTR·Alexandra R VaughnRaja K Sivamani
Oct 27, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Agata PanethPiotr Paneth
Sep 12, 2018·Nutrients·Noura S Dosoky, William N Setzer

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Citations

Apr 25, 2020·International Journal of Environmental Research and Public Health·Andrzej PolanczykZdzislaw Salamonowicz
Jul 27, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Ilona MandrikaTatjana Tracevska
Aug 8, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Meng WangHaixue Kuang

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