Antimicrobial Resistance and Molecular Epidemiology of Escherichia coli Causing Bloodstream Infections in Three Hospitals in Shanghai, China

PloS One
Su WangLizhong Han

Abstract

Escherichia coli (E. coli) is one of the most frequent and lethal causes of bloodstream infections (BSIs). We carried out a retrospective multicenter study on antimicrobial resistance and phylogenetic background of clinical E. coli isolates recovered from bloodstream in three hospitals in Shanghai. E. coli isolates causing BSIs were consecutively collected between Sept 2013 and Sept 2014. Ninety isolates randomly selected (30 from each hospital) were enrolled in the study. Antimicrobial susceptibility testing was performed by disk diffusion. PCR was used to detect antimicrobial resistance genes coding for β-lactamases (TEM, CTX-M, OXA, etc.), carbapenemases (IMP, VIM, KPC, NDM-1 and OXA-48), and phylogenetic groups. eBURST was applied for analysis of multi-locus sequence typing (MLST). The resistance rates for penicillins, second-generation cephalosporins, fluoroquinolone and tetracyclines were high (>60%). Sixty-one of the 90 (67.8%) strains enrolled produced ESBLs and no carbapenemases were found. Molecular analysis showed that CTX-M-15 (25/61), CTX-M-14 (18/61) and CTX-M-55 (9/61) were the most common ESBLs. Phylogenetic group B2 predominated (43.3%) and exhibited the highest rates of ESBLs production. ST131 (20/90) was the ...Continue Reading

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Citations

Sep 7, 2016·Virulence·David van Duin, Yohei Doi
Jun 14, 2019·Clinical Microbiology Reviews·Amee R MangesJohann D D Pitout
May 26, 2017·The Journal of Antimicrobial Chemotherapy·Edward R BevanPeter M Hawkey
Sep 5, 2017·World Journal of Clinical Cases·Fatema AlhashemMehmet Doganay
Dec 15, 2020·Archives of Gerontology and Geriatrics·Qiang ChenYan Jiang
Mar 18, 2021·International Journal of Microbiology·Svetlana I KolesnichenkoLyudmila L Akhmaltdinova
Mar 24, 2021·Antimicrobial Agents and Chemotherapy·Viivi H A HirvonenMarc W van der Kamp
Jan 2, 2021·Microbiology Spectrum·Lee W Riley

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Methods Mentioned

BETA
PCR

Software Mentioned

SAS
eBURST
EARS
Net

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