Antimicrobial resistance in Brachyspira pilosicoli with special reference to point mutations in the 23S rRNA gene associated with macrolide and lincosamide resistance

Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease
M KarlssonA Franklin

Abstract

A point mutation in the 23S rRNA gene causes macrolide and lincosamide resistance in Brachyspira hyodysenteriae. The possible occurrence of a similar mutation in Brachyspira pilosicoli was studied and the MICs of six antimicrobial agents for Swedish field isolates of B. pilosicoli were determined. Of 10 isolates with high MICs of macrolide and lincosamide antibiotics, six had a mutation in nucleotide position 2058 or 2059 in the 23S rRNA gene as compared to the wild type of Escherichia coli, whereas none of 10 tylosin-susceptible isolates were mutated in this region. The mutations found in position 2058 were A --> T transversions, and in position 2059 either A --> G transitions or A --> C transversions. The MICs at which 90% of the B. pilosicoli field isolates were inhibited by tylosin, erythromycin, clindamycin, virginiamycin, tiamulin, and carbadox, were >256, >256, >4, 4, 2, and 0.125 microg/ml, respectively. In conclusion, point mutations in positions 2058 and 2059 of the 23S rRNA gene can cause macrolide and lincosamide resistance in B. pilosicoli. Macrolide resistance is widespread among Swedish field isolates of B. pilosicoli. Notably also a few isolates with elevated MICs of tiamulin were found.

References

May 1, 1994·Antimicrobial Agents and Chemotherapy·G A Weinberg
Feb 1, 1994·Journal of Bacteriology·R L Zuerner, T B Stanton
May 1, 1994·Journal of Medical Microbiology·J I Lee, D J Hampson
Feb 1, 1994·Antimicrobial Agents and Chemotherapy·A MeierE C Böttger
Jul 1, 1995·Research in Veterinary Science·C Fellström, A Gunnarsson
Jan 1, 1996·International Journal of Systematic Bacteriology·D J TrottD J Hampson
Dec 1, 1995·Antimicrobial Agents and Chemotherapy·T S LucierP C Hu
Feb 1, 1996·Antimicrobial Agents and Chemotherapy·J VersalovicM F Go
Feb 1, 1997·Journal of Clinical Microbiology·C FellströmK E Johansson
Oct 24, 1998·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·G E DuhamelD Walter
Feb 19, 2002·Antimicrobial Agents and Chemotherapy·Marja PihlajamäkiJari Jalava
Mar 12, 2002·FEMS Microbiology Letters·Si Young LeeJ Christopher Fenno
Jun 7, 2003·Journal of Clinical Microbiology·M KarlssonA Franklin
Nov 5, 2003·Epidemiology and Infection·M FossiiS Pelkonen

❮ Previous
Next ❯

Citations

Jun 15, 2011·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Marc VerlindenAn Martel
Oct 7, 2009·Antimicrobial Agents and Chemotherapy·Lola V Stamm
Sep 27, 2008·Annals of Clinical Microbiology and Antimicrobials·Lilia Bait-MerabetVincent Cattoir
May 15, 2013·International Journal of Environmental Research and Public Health·Avelino Alvarez-OrdóñezPedro Rubio
May 6, 2014·The Journal of Antimicrobial Chemotherapy·Engeline van DuijkerenKarolina Törneke
Oct 29, 2005·Research in Veterinary Science·M PringleA Franklin
Apr 2, 2014·The Veterinary Journal·Satu PyöräläKarolina Törneke
Mar 12, 2016·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Nandita S Mirajkar, Connie J Gebhart
Mar 23, 2017·Canadian Journal of Microbiology·D G R S Kulathunga, J E Rubin
Mar 1, 2012·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Kristin A ClothierKent Schwartz
Dec 1, 2017·Clinical Microbiology Reviews·David J Hampson
Jun 3, 2016·Journal of Clinical Microbiology·Nandita S MirajkarConnie J Gebhart

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

CRISPR Screens in Drug Resistance

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on the application of CRISPR-Cas system in high-throughput genome-wide screens to identify genes that may confer drug resistance.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.