Antimicrobial resistance, integron carriage, and gyrA and gyrB mutations in Pseudomonas aeruginosa isolated from dogs with otitis externa and pyoderma in Brazil

Veterinary Dermatology
Lavicie R AraisAloysio M F Cerqueira

Abstract

Pseudomonas aeruginosa is associated with otitis and pyoderma in dogs and is frequently resistant to several antimicrobial drugs. Resistance genes can be carried by integrons with quinolone resistance mainly due to mutations in DNA topoisomerases II and IV. To evaluate the antimicrobial susceptibility, integron carriage, and gyrA and gyrB mutations in P. aeruginosa isolates from canine otitis and pyoderma. One hundred and four P. aeruginosa strains isolated from dogs with otitis externa (n = 93) and pyoderma (n = 11). Antimicrobial susceptibility against 16 antibacterial agents was evaluated through agar diffusion tests. Integron carriage, class and gyrA and gyrB mutations were analysed by PCR, restriction fragment length polymorphism (RFLP)-PCR and genetic sequencing assays. Isolates were mostly resistant to enrofloxacin (72.2%) and ticarcillin (59.7%). Lower resistance to ciprofloxacin (7.7%), tobramycin (3.8%) and polymixin B (0.0%) was detected. Ten (9.6%) multidrug-resistant (MDR) strains were detected. Eight (7.7%) strains carried class 1 integrons and this was associated with MDR (three isolates, P ≤ 0.05). Five of the integron-carrying strains exhibited aminoglycoside resistance genes. Mutations of gyrA and gyrB were ob...Continue Reading

References

Oct 1, 1994·Journal of Bacteriology·R M HallH W Stokes
Dec 31, 1998·Antimicrobial Agents and Chemotherapy·H MouneimnéE Cambau
Jan 6, 2000·FEMS Microbiology Letters·P A WhiteW D Rawlinson
Sep 13, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D C Hooper
Jun 28, 2003·Veterinary Microbiology·María Teresa TejedorJordi Vila
Dec 10, 2003·Journal of Clinical Microbiology·S ZhaoR D Walker
Apr 3, 2004·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A C Fluit, F-J Schmitz
Apr 6, 2004·The Veterinary Clinics of North America. Small Animal Practice·Daniel O Morris
May 24, 2005·FEMS Immunology and Medical Microbiology·Erica L FonsecaAna C P Vicente
Jul 24, 2007·The Journal of Antimicrobial Chemotherapy·Karl PedersenOle E Heuer
Nov 3, 2007·Journal of the American Animal Hospital Association·Lindsay McKayJack M Gaskin
Jun 17, 2009·Journal of Medical Microbiology·Tanya Strateva, Daniel Yordanov
Dec 25, 2009·FEMS Immunology and Medical Microbiology·Fereshteh ShahcheraghiMohammad M Feizabadi
Nov 26, 2010·Antimicrobial Agents and Chemotherapy·Mei-Feng LeeHan-Siong Toh
Feb 22, 2012·Frontiers in Microbiology·Laurent PoirelPatrice Nordmann
Jul 4, 2012·Antimicrobial Agents and Chemotherapy·Paula Regina Luna de Araújo JácomeMaria Amélia Vieira Maciel

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Citations

Sep 2, 2016·Veterinary Dermatology·Stefan SchwarzKristina Kadlec
May 28, 2019·BMC Veterinary Research·Milena TreschMichael Walkenhorst
Jun 8, 2017·Antibiotics·Maria Angeles ArgudínSandrine Roisin
Jul 2, 2021·EFSA Journal·UNKNOWN EFSA Panel on Animal Health and Welfare (AHAW)Julio Alvarez
Aug 14, 2018·Microbiology Spectrum·Agnese LupoJean-Yves Madec

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