Antimicrobial resistance of coagulase-negative staphylococci from bovine subclinical mastitis with particular reference to macrolide-lincosamide resistance phenotypes and genotypes

The Journal of Antimicrobial Chemotherapy
Petra Lüthje, Stefan Schwarz

Abstract

The aim of this study was to analyse coagulase-negative staphylococci (CoNS) for their resistance to antimicrobial agents approved for the control of pathogens involved in bovine mastitis, with particular reference to macrolide and/or lincosamide (ML) resistance and the resistance genes involved. A total of 298 CoNS collected between 2003 and 2005 in Germany from cases of subclinical mastitis in dairy cows were identified to the species level and investigated for their MICs by broth microdilution. ML-resistant isolates were subjected to plasmid profiling and electrotransformation experiments. The ML resistance genes were detected using PCR and hybridization. Selected PCR products were cloned and sequenced. The CoNS isolates used in this study showed a low level of resistance to all antimicrobial agents tested (0-7.4%) except ampicillin (18.1%). In the erythromycin-resistant and/or pirlimycin-resistant isolates, the ML resistance genes erm(B), erm(C), msr(A), mph(C) and lnu(A) were present, either alone or in different combinations. Isolates carrying erm methylase genes or the exporter gene msr(A) showed higher MICs than those harbouring only the genes mph(C) or lnu(A) coding for inactivating enzymes. Most of the ML resistance g...Continue Reading

References

Jan 27, 1999·FEMS Microbiology Letters·L B JensenF M Aarestrup
Jul 2, 1999·Antimicrobial Agents and Chemotherapy·C WerckenthinH Westh
Dec 3, 1999·Antimicrobial Agents and Chemotherapy·M C RobertsH Seppala
Oct 5, 2002·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Franz-Josef SchmitzStefan Schwarz
Jun 28, 2005·The Journal of Antimicrobial Chemotherapy·Tomasz HauschildStefan Schwarz

❮ Previous
Next ❯

Citations

Jun 8, 2010·The Journal of Antimicrobial Chemotherapy·Andrea T FesslerStefan Schwarz
Sep 30, 2010·Antimicrobial Agents and Chemotherapy·Andrea T FesslerStefan Schwarz
Dec 19, 2007·Antimicrobial Agents and Chemotherapy·Efthymia PetinakiRoland Leclercq
Oct 20, 2012·PloS One·Georgios OikonomouRodrigo Carvalho Bicalho
Jan 28, 2014·PloS One·Georgios OikonomouRodrigo Carvalho Bicalho
Jun 1, 2011·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Jae-Ik HanKi-Jeong Na
Oct 12, 2007·Expert Opinion on Pharmacotherapy·David Guay
May 7, 2013·Enfermedades infecciosas y microbiología clínica·Bruna LeiteRosário Oliveira
Oct 1, 2011·Veterinary Microbiology·Marinella Silva LaportMarcia Giambiagi-deMarval
May 13, 2011·Veterinary Microbiology·K Persson WallerU Grönlund Andersson
Jan 7, 2016·Veterinary Microbiology·Monika EntorfStefan Schwarz
Jul 14, 2010·Veterinary Microbiology·Tomasz Hauschild, Stefan Schwarz
Sep 14, 2015·Journal of Dairy Science·Maged El-AshkerHelmut Hotzel
Mar 16, 2016·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Anna Lenart-BorońAndrzej Kasprowic
Jan 28, 2014·Veterinary Microbiology·Monika EntorfStefan Schwarz
Mar 19, 2013·International Journal of Medical Microbiology : IJMM·Sarah WendlandtKristina Kadlec
Jul 3, 2013·The Journal of Antimicrobial Chemotherapy·François GraveyVincent Cattoir
Dec 28, 2010·International Journal of Antimicrobial Agents·Anne Le BouterVincent Cattoir
Apr 2, 2014·The Veterinary Journal·Satu PyöräläKarolina Törneke

❮ Previous
Next ❯

Related Concepts

Related Feeds

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.