PMID: 9423225Jan 10, 1998Paper

Antimicrobial resistance testing of Helicobacter pylori: a comparison of Etest and disk diffusion methods

Pathology
P D MidoloM L Grayson

Abstract

Routine antimicrobial resistance testing of Helicobacter pylori is more commonly performed since the correlation between metronidazole resistance and failure to eradicate using this drug, has been made. While resistance testing of H. pylori by Etest is simple to perform, it is expensive compared to disk diffusion methods. In this study the Etest was compared with a modified Kirby-Bauer disk diffusion (NCCLS) method for routine resistance screening of H. pylori. Fifty one pre-treatment isolates were tested against amoxycillin, metronidazole, tetracycline and erythromycin by both Etest and disk diffusion using NCCLS guideline strength disks. Clarithromycin was tested by Etest only. Nitroimidazole and macrolide resistance were detected using the modified Kirby-Bauer disk diffusion method which correlated with Etest minimum inhibitory concentration (MIC). Resistance rates were 49% for metronidazole and 8% for clarithromycin. Cross resistance occurs with macrolides against H. pylori and allows testing of erythromycin to predict resistance to clarithromycin. The very low MICs obtained with H. pylori against amoxycillin and tetracycline require the use of Etest or lower strength disk methods to be used.

References

Dec 1, 1974·The Journal of Infectious Diseases·C M Metzler, R M DeHaan
Apr 27, 1996·Lancet·P D MidoloJ R Lambert
Mar 1, 1996·Journal of Gastroenterology and Hepatology·P D MidoloJ Turnidge

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Citations

Jan 31, 2003·Journal of Molecular Recognition : JMR·K T MomynalievA I Archakov
Jan 13, 2000·Alimentary Pharmacology & Therapeutics·E J Van Der WoudenJ H Kleibeuker
Jun 26, 2001·Symposium Series·J G Kusters, E J Kuipers
Dec 6, 2000·Journal of Gastroenterology and Hepatology·K M Fock
Jul 31, 2003·Journal of Paediatrics and Child Health·S RerksuppapholP Ward
Mar 23, 2002·The Journal of Antimicrobial Chemotherapy·Cliodna McNultyUNKNOWN PHLS Helicobacter Working Group
Jun 27, 2000·Australian and New Zealand Journal of Medicine·A G FraserB Hollis
Oct 10, 2003·Arquivos De Gastroenterologia·Jaime Natan EisigAntonio Atilio Laudanna
Feb 1, 2005·World Journal of Gastroenterology : WJG·Selim AydemirUgur Yilmaz
Jul 31, 2019·The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien Des Maladies Infectieuses Et De La Microbiologie Médicale·Youn I ChoiSun-Mi Lee
Jan 31, 2002·World Journal of Gastroenterology : WJG·Hua-Xiang XiaNicholas J Talley
Nov 21, 2020·Journal of Gastroenterology and Hepatology·Jonathon P SchubertRobert V Bryant
Feb 24, 2001·The Medical Journal of Australia·L C MollisonD B McGechie

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