Antimicrobial Susceptibility and Characterization of Resistance Mechanisms of Corynebacteriumurealyticum Clinical Isolates

Antibiotics
Itziar Chapartegui-GonzálezJesús Navas

Abstract

Corynebacterium urealyticum is a non-diphtherial urease-producing clinically relevant corynebacterial, most frequently involved in urinary tract infections. Most of the C. urealyticum clinical isolates are frequently resistant to several antibiotics. We investigated the susceptibility of 40 C. urealyticum isolated in our institution during the period 2005-2017 to eight compounds representative of the main clinically relevant classes of antimicrobial agents. Antimicrobial susceptibility was determined by the Epsilometer test. Resistance genes were searched by PCR. All strains were susceptible to vancomycin whereas linezolid and rifampicin also showed good activity (MICs90 = 1 and 0.4 mg/L, respectively). Almost all isolates (39/40, 97.5%) were multidrug resistant. The highest resistance rate was observed for ampicillin (100%), followed by erythromycin (95%) and levofloxacin (95%). Ampicillin resistance was associated with the presence of the blaA gene, encoding a class A β-lactamase. The two rifampicin-resistant strains showed point mutations driving amino acid replacements in conserved residues of RNA polymerase subunit β (RpoB). Tetracycline resistance was due to an efflux-mediated mechanism. Thirty-nine PFGE patterns were ide...Continue Reading

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Datasets Mentioned

BETA
VH3073
VH4248
VH5757
VH5913

Methods Mentioned

BETA
PCR
Electrophoresis

Software Mentioned

EDGAR
coffee
SPAdes assembler
Fingerprinting II
MEGA7
PATRIC
RAST
Clustal W

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