Antimicrobial susceptibility assays based on the quantification of bacterial lipopolysaccharides via a label free lectin biosensor

Analytical Chemistry
Fen MaXiangqun Zeng

Abstract

A label free lectin biosensor developed in our laboratory that can quantitatively measure the binding between the lectin immobilized at the carbohydrate sensor surface and the lipopolysaccharide (LPS) on Gram-negative bacteria was demonstrated for an antibiotic susceptibility assay. The biosensor utilizes a polythiophene interface containing fused quinone moieties glycosylated to form a carbohydrate platform for the immobilization of Concanavalin A (Con A) and is capable of LPS binding measurements via orthogonal quartz crystal microbalance and electrochemical readouts (EQCM). Such orthogonal transduction provides cross-validation, better sensor sensitivity, and a large dynamic range of the measurements. We have applied this label free lectin biosensor for a new antibiotic susceptibility assay by characterizing the antimicrobial activities of various antibiotics (i.e., ciprofloxacin, ceftriaxone, and tetracycline) against Escherichia coli W1485 as a model system. The label free biosensor allows both end point and real time measurements of antibiotic effects on the bacterial cell surface LPS, which is shown to correlate to their antibiotic effects. At the end point, after 18 h incubation of bacterial cells with these three antib...Continue Reading

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Citations

Mar 4, 2014·Current Opinion in Microbiology·Huan-Yuan ChenFu-Tong Liu
Aug 16, 2016·Accounts of Chemical Research·Xiangqun ZengAbdul Rehman
Dec 6, 2016·Sensors·Uichi Akiba, Jun-Ichi Anzai
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Dec 9, 2015·Materials·Baozhen Wang, Jun-Ichi Anzai
Aug 20, 2019·Biosensors & Bioelectronics·Bhagaban BeheraHardik J Pandya

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