Abstract
Antimicrobial susceptibility testing for Helicobacter pylori is increasingly important due to resistance to the most used antimicrobials agents. Only agar dilution method is approved by CLSI, but it is difficult to perform routinely. We evaluated the reliability of E-test and disk diffusion comparing to agar dilution method on Helicobacter pylori antimicrobial susceptibility testing. Susceptibility testing was performed for amoxicillin, clarithromycin, furazolidone, metronidazole and tetracycline using E-test, disk-diffusion and agar dilution method in 77 consecutive Helicobacter pylori strains from dyspeptic children and adolescents. Resistance rates were: amoxicillin - 10.4%, 9% and 68.8%; clarithromycin - 19.5%, 20.8%, 36.3%; metronidazole - 40.2%33.7%, 38.9%, respectively by agar dilution, E-test and disk diffusion method. Furazolidone and tetracycline showed no resistance rates. Metronidazole presented strong correlation to E-test (r = 0.7992, p < 0.0001) and disk diffusion method (r=-0.6962, p < 0.0001). Clarithromycin presented moderate correlation to E-test (r = 0.6369, p < 0.0001) and disk diffusion method (r=-0.5656, p < 0.0001). Amoxicillin presented weak correlation to E-test (r = 0.3565, p = 0.0015) and disk diffus...Continue Reading
Citations
Mar 28, 2019·PloS One·Muhammad MiftahussururYoshio Yamaoka
May 27, 2020·Helicobacter·Xiaoqiong TangHong Tang
May 21, 2017·Biomédica : revista del Instituto Nacional de Salud·Claudia Patricia AcostaAlba Alicia Trespalacios
Nov 1, 2017·World Journal of Gastroenterology : WJG·Mohammad ZamaniJavad Shokri-Shirvani
Jan 15, 2019·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Changcheng GuoZhenghong Chen
Mar 1, 2019·Antimicrobial Resistance and Infection Control·Muhammad MiftahussururYoshio Yamaoka
May 23, 2018·Paediatric Drugs·Kallirroi KotileaPatrick Bontems
Aug 10, 2018·Helicobacter·Gisela M SilvaRosa Lima
Jan 11, 2019·PloS One·Andrew A Heller, Dana M Spence
Nov 14, 2019·Arquivos De Gastroenterologia·Cristian Parra-SepúlvedaApolinaria García-Cancino
Aug 7, 2019·Scientific Reports·Andrés GonzálezÁngel Lanas
Sep 10, 2020·Future Microbiology·Xiaoqiong TangHong Tang
Aug 20, 2016·Jundishapur Journal of Microbiology·Mojdeh Hakemi ValaMehrdad Gholami
Jul 22, 2018·Infectious Agents and Cancer·Doaa M Al-ErakyMohammed A Ramadan
May 20, 2017·World Journal of Gastroenterology : WJG·Nazlı ArslanEbru Demiray-Gürbüz
Jan 12, 2020·BMC Research Notes·Muhammad MiftahussururYoshio Yamaoka
Jun 12, 2021·Journal of Gastroenterology and Hepatology·Rachel Gingold-BelferDoron Boltin
Jul 3, 2021·Microorganisms·Xi WangMorgan B Farnell