Antimicrobial susceptibility testing for Helicobacter pylori isolates from Brazilian children and adolescents: comparing agar dilution, E-test, and disk diffusion

Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]
Silvio K OgataElisabete Kawakami

Abstract

Antimicrobial susceptibility testing for Helicobacter pylori is increasingly important due to resistance to the most used antimicrobials agents. Only agar dilution method is approved by CLSI, but it is difficult to perform routinely. We evaluated the reliability of E-test and disk diffusion comparing to agar dilution method on Helicobacter pylori antimicrobial susceptibility testing. Susceptibility testing was performed for amoxicillin, clarithromycin, furazolidone, metronidazole and tetracycline using E-test, disk-diffusion and agar dilution method in 77 consecutive Helicobacter pylori strains from dyspeptic children and adolescents. Resistance rates were: amoxicillin - 10.4%, 9% and 68.8%; clarithromycin - 19.5%, 20.8%, 36.3%; metronidazole - 40.2%33.7%, 38.9%, respectively by agar dilution, E-test and disk diffusion method. Furazolidone and tetracycline showed no resistance rates. Metronidazole presented strong correlation to E-test (r = 0.7992, p < 0.0001) and disk diffusion method (r=-0.6962, p < 0.0001). Clarithromycin presented moderate correlation to E-test (r = 0.6369, p < 0.0001) and disk diffusion method (r=-0.5656, p < 0.0001). Amoxicillin presented weak correlation to E-test (r = 0.3565, p = 0.0015) and disk diffus...Continue Reading

Citations

May 21, 2017·Biomédica : revista del Instituto Nacional de Salud·Claudia Patricia AcostaAlba Alicia Trespalacios
Nov 1, 2017·World Journal of Gastroenterology : WJG·Mohammad ZamaniJavad Shokri-Shirvani
Jan 15, 2019·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Changcheng GuoZhenghong Chen
Mar 1, 2019·Antimicrobial Resistance and Infection Control·Muhammad MiftahussururYoshio Yamaoka
Aug 10, 2018·Helicobacter·Gisela M SilvaRosa Lima
Jan 11, 2019·PloS One·Andrew A Heller, Dana M Spence
Nov 14, 2019·Arquivos De Gastroenterologia·Cristian Parra-SepúlvedaApolinaria García-Cancino
Jul 22, 2018·Infectious Agents and Cancer·Doaa M Al-ErakyMohammed A Ramadan
May 20, 2017·World Journal of Gastroenterology : WJG·Nazlı ArslanEbru Demiray-Gürbüz
Jun 12, 2021·Journal of Gastroenterology and Hepatology·Rachel Gingold-BelferDoron Boltin

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