PMID: 9420032Jan 7, 1998Paper

Antimicrobial susceptibility testing of 230 Helicobacter pylori strains: importance of medium, inoculum, and incubation time

Antimicrobial Agents and Chemotherapy
S H HartzenO Bonnevie

Abstract

No standardized method of susceptibility testing for Helicobacter pylori is currently available, so before a large agar dilution study comprising 230 H. pylori strains belonging to more than 80 genetically different groups was initiated, we performed a relatively small preliminary study to determine the influences of medium, inoculum density, and incubation time. Seven media were investigated and were primarily evaluated on the basis of their abilities to support growth both semiquantitatively and qualitatively; Iso-Sensitest agar supplemented with 10% horse blood was found to be well suited for the purpose; this was closely followed by Mueller-Hinton agar with 10% horse blood, Mueller-Hinton with 10% sheep blood, and finally, 7% lysed horse blood agar. Investigations of two inoculum densities and two incubation times resulted in recommendations for the use of 10(9) CFU/ml (10[6] CFU/spot) as the inoculum and 72 h as the incubation time. A modest inoculum effect was noted for amoxicillin and metronidazole. By the methodology derived from our preliminary study, the susceptibilities of 230 H. pylori strains to six antibiotics were subsequently determined. The results were generally in accord with those of others, and apart from m...Continue Reading

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Citations

May 11, 2002·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·B GrignonJ L Fauchère
Jun 28, 2005·Antimicrobial Agents and Chemotherapy·K Van den BulckF Haesebrouck
Nov 20, 2009·Journal of Applied Microbiology·J R SoberónM A Vattuone
Dec 24, 2015·Alimentary Pharmacology & Therapeutics·I ThungM A Valasek
Mar 18, 2000·Journal of Chemotherapy·F BazzoliE Roda
Jan 13, 2000·Alimentary Pharmacology & Therapeutics·E J Van Der WoudenJ H Kleibeuker
Jun 19, 2002·Antimicrobial Agents and Chemotherapy·N CíftçíogluE O Kajander
Jun 23, 1999·FEMS Immunology and Medical Microbiology·S BereswillM Kist
Jun 23, 1999·FEMS Immunology and Medical Microbiology·H ColdingK A Krogfelt
May 1, 1999·Antimicrobial Agents and Chemotherapy·J K AkadaT Nakazawa
Oct 30, 1999·Antimicrobial Agents and Chemotherapy·F MégraudC F Burman
Apr 15, 2003·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·H ColdingL P Andersen
Aug 20, 2003·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·R J L F Loffeld, C A P M J Fijen

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