Antimycoplasma properties and application in cell culture of surfactin, a lipopeptide antibiotic from Bacillus subtilis.

Applied and Environmental Microbiology
D VollenbroichJ Vater

Abstract

Surfactin, a cyclic lipopeptide antibiotic and biosurfactant produced by Bacillus subtilis, is well-known for its interactions with artificial and biomembrane systems (e.g., bacterial protoplasts or enveloped viruses). To assess the applicability of this antiviral and antibacterial drug, we determined the cytotoxicity of surfactin with a 50% cytotoxic concentration of 30 to 64 microM for a variety of human and animal cell lines in vitro. Concomitantly, we observed an improvement in proliferation rates and changes in the morphology of mycoplasma-contaminated mammalian cells after treatment with this drug. A single treatment over one passage led to complete removal of viable Mycoplasma hyorhinis cells from various adherent cell lines, and Mycoplasma orale was removed from nonadherent human T-lymphoid cell lines by double treatment. This effect was monitored by a DNA fluorescence test, an enzyme-linked immunosorbent assay, and two different PCR methods. Disintegration of the mycoplasma membranes as observed by electron microscopy indicated the mode of action of surfactin. Disintegration is obviously due to a physicochemical interaction of the membrane-active surfactant with the outer part of the lipid membrane bilayer, which cause...Continue Reading

References

Jun 1, 1978·Microbiological Reviews·S Razin
Apr 27, 1992·Journal of Immunological Methods·C C UphoffH G Drexler
Apr 26, 1991·Biochimica Et Biophysica Acta·J D SheppardR Laprade
Jun 28, 1991·Biochemical and Biophysical Research Communications·F BaumgartD Ziessow
Jun 8, 1989·Nature·R J HayT R Chen
Jun 1, 1970·Journal of General Microbiology·A W Bernheimer, L S Avigad
Mar 30, 1984·Journal of Immunological Methods·D A Flick, G E Gifford
Jan 1, 1994·Applied and Environmental Microbiology·F J van KuppeveldW J Melchers
May 1, 1995·Biophysical Journal·R Maget-Dana, M Ptak
Jan 1, 1994·Annual Review of Microbiology·A Blanchard, L Montagnier
Jan 1, 1994·Applied and Environmental Microbiology·S C LinG Georgiou

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Citations

Jul 15, 2006·Molecular Plant-microbe Interactions : MPMI·Jos M RaaijmakersMaarten J D de Kock
Oct 20, 2006·European Biophysics Journal : EBJ·Heiko Heerklotz, Joachim Seelig
Apr 13, 2007·Molecular Plant-microbe Interactions : MPMI·Diego RomeroAlejandro Pérez-García
Apr 24, 2010·FEMS Microbiology Reviews·Jos M RaaijmakersMarc Ongena
Jan 9, 2016·Current Microbiology·Anila Fariq, Ayesha Saeed
Apr 23, 1999·Journal of Applied Microbiology·A M AnderssonM S Salkinoja-Salonen
Jun 24, 2000·European Journal of Biochemistry·R MikkolaM S Salkinoja-Salonen
Nov 28, 2002·Journal of Applied Microbiology·N Christofi, I B Ivshina
Jun 6, 2003·Applied and Environmental Microbiology·Adria A BodourRaina M Maier
Apr 4, 2002·In Vitro Cellular & Developmental Biology. Animal·Cord C Uphoff, Hans G Drexler
Jul 21, 2007·Magnetic Resonance in Chemistry : MRC·Jin-Shan TangXin-Sheng Yao
Feb 14, 2006·The Journal of Antimicrobial Chemotherapy·Lígia RodriguesRosário Oliveira
Apr 26, 2008·Applied Biochemistry and Biotechnology·Ahmad Mohammad Abdel-MawgoudNadia Abdel-Haleem Hassouna
Oct 3, 2007·Journal of Industrial Microbiology & Biotechnology·Benjamín Otto Ortega-MoralesTorsten Stein
Aug 7, 2008·Applied Biochemistry and Biotechnology·A Mohammad Abdel-MawgoudNadia Abdel-Haleem Hassouna
Jan 6, 2011·FEMS Microbiology Letters·Olfa TabbeneFerid Limam
Jul 25, 2012·Proceedings of the National Academy of Sciences of the United States of America·B Christopher HoeflerPaul D Straight
Sep 14, 2019·Applied Microbiology and Biotechnology·Aline Wasem ZanottoGláucia Maria Pastore
Jan 18, 2002·Antimicrobial Agents and Chemotherapy·Norimasa TamehiroKozo Ochi
Nov 6, 2009·International Journal of Cosmetic Science·M Kanlayavattanakul, N Lourith
May 29, 2014·Medicinal Research Reviews·Stephen A Cochrane, John C Vederas
Feb 13, 2016·Environmental Science and Pollution Research International·Thando NdlovuWesaal Khan
Jan 30, 2015·BioMed Research International·Khem Raj Meena, Shamsher S Kanwar
Apr 11, 2018·Avian Pathology : Journal of the W.V.P.A·Ar'Quette GrantHyun S Lillehoj
Jun 22, 2019·Microbiology Resource Announcements·Stine Sofie Frank NielsenAndreas Schramm
Jul 14, 2010·Journal of Mass Spectrometry : JMS·Ylenia PecciGianna Allegrone
Jul 26, 2008·Archives of Microbiology·Augusto EtchegarayOmar Teschke
Oct 4, 2007·Pest Management Science·Yun Sung KimBeom Seok Kim
Jan 13, 2015·Applied Microbiology and Biotechnology·Clémence LoiseauJulien Verdon

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