Antineoplastic effects of clove buds (Syzygium aromaticum L.) in the model of breast carcinoma

Journal of Cellular and Molecular Medicine
Peter KubatkaDietrich Büsselberg

Abstract

It is supposed that plant functional foods, rich in phytochemicals, may potentially have preventive effects in carcinogenesis. In this study, the anticancer effects of cloves in the in vivo and in vitro mammary carcinoma model were assessed. Dried flower buds of cloves (CLOs) were used at two concentrations of 0.1% and 1% through diet during 13 weeks after the application of chemocarcinogen. After autopsy, histopathological and immunohistochemical analyses of rat mammary carcinomas were performed. Moreover, in vitro evaluation using MCF-7 cells was carried out. Dietary administered CLO caused the dose-dependent decrease in tumour frequency by 47.5% and 58.5% when compared to control. Analysis of carcinoma cells in animals showed bcl-2, Ki67, VEGFA, CD24 and CD44 expression decrease and Bax, caspase-3 and ALDH1 expression increase after high-dose CLO administration. MDA levels were substantially decreased in rat carcinomas in both CLO groups. The evaluation of histone modifications revealed increase in lysine trimethylations and acetylations (H4K20me3, H4K16ac) in carcinomas after CLO administration. TIMP3 promoter methylation levels of CpG3, CpG4, CpG5 islands were altered in treated cancer cells. An increase in total RASSF1A p...Continue Reading

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Citations

Mar 7, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alena LiskovaDietrich Büsselberg
Apr 12, 2019·International Journal of Molecular Sciences·Peter KubatkaDietrich Büsselberg
Apr 28, 2019·Cells·Natalia A Riobo-Del GaldoEva V Wertheimer
Jan 14, 2020·Journal of Chemotherapy·Xue-Jun WangMei-Juan Yan
May 28, 2019·Journal of Cancer Research and Clinical Oncology·Marek SamecDietrich Büsselberg
Dec 12, 2018·Plant Foods for Human Nutrition·Lee Kee ChuaRhun Yian Koh
Mar 25, 2019·Journal of Cancer Research and Clinical Oncology·Andrea KapinovaJan Danko
Nov 30, 2018·Journal of Experimental & Clinical Cancer Research : CR·Guanming LuZhongheng Wei
Mar 25, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Peter KubatkaJan Mojzis
Dec 31, 2020·International Journal of Molecular Sciences·Peter KubatkaJan Mojzis
Mar 5, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Mariam AbotalebDietrich Büsselberg

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Methods Mentioned

BETA
MDA
PCR
electrophoresis
FCS
Assay
ELISA
flow cytometry
acetylation

Software Mentioned

GraphPad
PyroMark Q96
QuickPHOTO MICRO
graphpad prism
R

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