Antinucleosome autoantibodies bind directly to cell lines in vitro and via the FcgammaRIIB receptor to B lymphocytes in vivo: a role for immune complexes in interactions between antinucleosome IgG2a and B cells of BXSB lupus mice

Scandinavian Journal of Immunology
X LiK Hannestad

Abstract

The initial novel observation of this study was that most B cells of male BXSB lupus mice bear surface IgG2a(b) of extrinsic origin. To define the surface antigen, we here examine three (NZBxBXSB)F1-derived IgG2a(b) monoclonal antibodies (mAbs) selected for binding to cell surfaces. Surprisingly, all three mAbs bound the nucleosome (nuc) particle, the fundamental unit of chromatin and an early target of autoimmunity in systemic lupus erythematosus. Their tentative dissociation constant (K(d)) for soluble nuc particles was approximately 7 x 10(-10) m. The mAbs bound more weakly to both H2A-H2B-DNA and H3-H4-DNA complexes, and in immunoblot they stained all four core histones. The mAbs detected a surface antigen on all cell lines examined, present on viable cells. When stripped of nuc, and in the presence of DNase I, their binding to cell lines improved. Heparin displaced the antigen from the cell surface. In vivo, the three mAbs stained B cells of several BALB/c mice clearly stronger than the isotype control; this differential staining was significantly reduced in FcgammaRIIB-deficient mice. The results indicate that the three mAbs recognize (a) planted antigen on viable cultured cells and (b) soluble autoantigen in vivo, leadin...Continue Reading

References

Oct 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·H M DintzisB Vogelstein
Oct 19, 1978·Nature·K Hannestad, B D Stollar
Feb 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·B R ShawK E Van Holde
Jul 1, 1990·The Journal of Clinical Investigation·P M Rumore, C R Steinman
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·L JacobJ F Bach
Nov 1, 1985·Scandinavian Journal of Immunology·P FaaberJ H Berden
Jun 1, 1987·Arthritis and Rheumatism·K OkudairaR C Williams
Sep 1, 1985·The Journal of Clinical Investigation·V M Holers, B L Kotzin
Jan 1, 1985·Immunology Letters·Y ShoenfeldJ Pinkhas
Dec 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·S Sirisinha, H N Eisen
Jan 1, 1968·Advances in Immunology·D W Dresser, N A Mitchison
Feb 1, 1993·European Journal of Immunology·E RazD Eilat
Apr 6, 1995·Nature·A M KriegD M Klinman
Apr 1, 1993·The Journal of Clinical Investigation·R W BurlingameA N Theofilopoulos
Jan 1, 1996·European Journal of Immunology·I KropD T Fearon
Dec 1, 1996·Journal of Autoimmunity·K KramersJ H Berden
Jul 27, 1999·The Journal of Biological Chemistry·K WatsonR J Edwards
Feb 13, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·X ZhangL J Wysocki
May 25, 2001·Nature·F D BatistaM S Neuberger
Jul 13, 2001·European Journal of Immunology·S K Reitan, K Hannestad
Apr 12, 2002·Nature·Elizabeth A LeadbetterAnn Marshak-Rothstein
Apr 27, 2002·Nature Immunology·Arthur M Krieg
Jun 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Brian A CoccaMarko Z Radic
Dec 4, 2002·Nature Reviews. Immunology·John SavillChris Haslett

❮ Previous
Next ❯

Citations

Nov 14, 2013·PLoS Biology·Chaohong LiuWenxia Song
Jan 2, 2007·Trends in Immunology·Marc Ehlers, Jeffrey V Ravetch
Jun 9, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kristian Hannestad, Helge Scott
Aug 7, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kristian Hannestad, Helge Scott

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.