Antiosteoporotic effects and proteomic characterization of the target and mechanism of an Er-Xian Decoction on osteoblastic UMR-106 and osteoclasts induced from RAW264.7.

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Zheng ZhuQiao-Yan Zhang

Abstract

Er-Xian Decoction (EXD) has been used for the treatment of osteoporosis disorders, menopausal syndrome, and other aging diseases in the Chinese traditional healthcare system. However, the targets and mechanism of action have not been clarified. This study was designed to investigate the effects and possible target proteins of EXD on osteoblastic UMR-106 cells and osteoclasts induced from RAW264.7 cells using the proteomic analysis technique. We found that EXD at a concentration of 50-200 microg/mL significantly enhanced osteoblastic UMR-106 cell proliferation, alkaline phosphatase (ALP) activity and formation of bone nodules, and decreased tartrate-resistant acid phosphatase (TRAP) activity and the bone resorption action of osteoclasts induced from RAW 264.7 cells. In EXD-treated osteoblasts, there were increases in the expression of heat-shock protein 1, high mobility group protein (Hmgb1), acidic ribosomal phosphoprotein P0, histone 2, carbonyl reductase 1, ATP synthase, aldolase A, and Rho GDP dissociation inhibitor (GDI)-alpha; and reduction in the expression of carbonic anhydrase 3, prohibitin, hemiferrin, far upstream element (FUSE)-binding protein. In EXD-treated osteoclasts, there were increases in the expression of vim...Continue Reading

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Citations

Jun 26, 2013·Menopause : the Journal of the North American Menopause Society·Linda L D ZhongBen X B Yu
Jun 5, 2013·Genome Medicine·Shuyan WuHong-Wen Deng
Apr 9, 2014·The American Journal of Chinese Medicine·Ka-Chun WongMan-Sau Wong
Apr 3, 2012·Expert Review of Proteomics·Annalisa CastagnaDomenico Girelli
Apr 16, 2013·Journal of Tissue Engineering and Regenerative Medicine·Werner E G MüllerXiaohong Wang
Jan 27, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiaoxue XuXueqin Ma
Apr 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiaohong WangWerner E G Müller

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electrophoresis
electrophoreses

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PDQuest7
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Leica
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