Antioxidant action of six Trifolium species in blood platelet experimental system in vitro

Molecular and Cellular Biochemistry
Joanna Kolodziejczyk-CzepasAnna Stochmal

Abstract

This study includes a comparative evaluation of antioxidant effects of plant extracts (1.5-50.0 μg/ml), derived from six clover (Trifolium) species: T. alexandrinum L., T. fragiferum L., T. hybridum L., T. incarnatum L., T. resupinatum var. majus Boiss., and T. resupinatum var. resupinatum L. Chemical profiles of the extracts contained three or four groups of (poly)phenolic compounds such as phenolic acids, clovamides, isoflavones, and other flavonoids. Antioxidant properties of Trifolium extracts were assessed as the efficacy to reduce oxidative and nitrative damage to blood platelets, exposed to 100 μM peroxynitrite-induced oxidative stress in vitro. Antioxidant actions of the examined extracts were determined by the following biomarkers of oxidative stress: thiol groups, 3-nitrotyrosine, lipid hydroperoxides, and thiobarbituric acid-reactive substances (TBARS). Despite the significant differences in the chemical composition (the total phenolic concentrations varied between 11.30 and 52.55 mg/g of dry mass) of Trifolium extracts, we observed noticeable protective effects of almost all tested plant preparations. The T. alexandrinum extract, containing the highest concentration of phenols, was the most effective antioxidant amo...Continue Reading

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Citations

May 28, 2016·The Journal of Pharmacy and Pharmacology·Joanna Kolodziejczyk-Czepas

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Methods Mentioned

BETA
ELISA
c-ELISA

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