Antioxidant activities of two metallothionein-like proteins from sweet potato (Ipomoea batatas [L.] Lam. `Tainong 57') storage roots and their synthesized peptides

Botanical Studies
Shyh-Shyun HuangGuan-Jhong Huang

Abstract

Metallothionein (MT) characterized by their low molecular weight and high cysteine content. Two recombinant proteins of MT-I and MT-II overproduced in E. coli (M15) was purified by Ni2+-chelated affinity chromatography. The molecular mass of MT-I and MT-II are ca. 6,600 and 8,000 Da as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Total antioxidant status, DPPH radical scavenging activity, reducing power method, Fe2+-chelating ability, ferric thiocyanate (FTC) method, and protecting calf thymus DNA against hydroxyl radical-induced damage were studied. The MT-I and MT-II proteins with a concentration of 100 μg/mL exhibited the highest activity (expressed respectively as 61.72 ± 0.13 and 74.28 ± 1.15 μM Trolox equivalent antioxidative capacity, TEAC) in total antioxidant status test. Like total antioxidant status, DPPH radical scavenging activity, reducing power, Fe2+-chelating ability, FTC activity, and protecting calf thymus DNA against hydroxyl radical-induced damage all showed that MT-1 and MT-II proteins have antioxidant activities. In this study, we also found that antioxidant activities of MT-I and MT-II increased from 17% and 16% (0 h) to about 26% and 28% (24 h) after 24 h hydrolysis...Continue Reading

References

Jan 4, 1994·Proceedings of the National Academy of Sciences of the United States of America·W Maret
Mar 1, 1993·Free Radical Biology & Medicine·M Sato, I Bremner
Jan 1, 1993·Critical Reviews in Toxicology·J P Kehrer
Aug 5, 2004·Journal of Agricultural and Food Chemistry·Yanping ZouDongzhi Wei
Oct 12, 2011·Current Opinion in Clinical Nutrition and Metabolic Care·Robert A McPherson, Gil Hardy

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Citations

Jan 22, 2017·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Paulami PramanickSarmistha Sen Raychaudhuri
May 17, 2021·Food Research International·Edilza Silva do NascimentoCarlos Alberto de Almeida Gadelha

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Methods Mentioned

BETA
PCR
dot-blot
electrophoresis
dot blot
dot blots

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