Antioxidant activity, acetylcholinesterase inhibitory potential and phytochemical analysis of Sarcocephalus latifolius Sm. bark used in traditional medicine in Sudan

BMC Complementary and Alternative Medicine
Alsiddig OsamaAmna Ali

Abstract

Sarcocephalus latifolius is used as a traditional medicine for curing many diseases in Sudan. The main objective of the current study was to determine the antioxidant activity and acetylcholinesterase inhibition (AChEI) of S. latifolius, and to estimate its total phenolic and flavonoid contents. Antioxidant activity of the tested plant extracts was carried out by determining their ability to scavenge the 2,2-diphenyl-1-picryl hydrazyl (DPPH) free radical. On the other hand, AChE inhibitory activity was determined spectrophotometrically using the Ellman's colorimetric method. The levels of total phenols and flavonoids were determined quantitatively using spectrophotometric methods. MTT assay was consumed to assess the cytotoxic effect of the most active fractions. These fractions were subjected to phytochemical analysis using GC-MS techniques to determine thier chemical composition. Hexane and chloroform fractions exhibited the highest antioxidant activity with IC50 values of (0.098 ± 0.08 and 0.099 ± 0.029 mg/ml) respectively. Standard propyl gallate had the lowest IC50 value of 0.0414 ± 0.11 mg/ml. The ethanolic crude extract showed low AChEI activity with 40.2 ± 0.10%. High concentrations of phenolic and flavonoid contents we...Continue Reading

References

Feb 1, 1992·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·O I AruomaJ Löligers
Jul 1, 1993·British Medical Bulletin·V R WinrowD R Blake
Apr 18, 2000·Archives of Neurology·G EmilienJ M Maloteaux
Jun 17, 2000·International Journal of Food Microbiology·J P RauhaP Vuorela
Jan 23, 2003·Journal of Agricultural and Food Chemistry·Kelly WolfeRui Hai Liu
May 10, 2003·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Z Sroka, W Cisowski
Dec 23, 2003·Current Medical Research and Opinion·Serge GauthierSteven G Potkin
Jul 24, 2004·Trends in Neurosciences·Robert G ShulmanFahmeed Hyder
Aug 6, 2004·Nature·Mark P Mattson
Jul 29, 2005·Critical Reviews in Food Science and Nutrition·Augustin ScalbertLiliana Jiménez
Dec 27, 2005·Molecular Immunology·M Ahmed MesaikM Iqbal Choudhary
Mar 10, 2007·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Pulok K MukherjeePeter J Houghton
Jan 1, 2008·Journal of Ethnopharmacology·Filomena ConfortiRoberto Della Loggia
Dec 23, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·F S SenolB Sener

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Jul 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nor Atiqah JusrilMohd Ilham Adenan

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