Antioxidant activity and phenolic composition in pomegranate (Punica granatum L.) genotypes from south Italy by UHPLC-Orbitrap-MS approach

Journal of the Science of Food and Agriculture
Vita Di StefanoMaria Grazia Melilli

Abstract

Pomegranate fruits are a rich source of polyphenols with numerous health-promoting effects. Pomegranate juices of five genotypes ('Mollar', 'Kingdom', 'Dente di Cavallo', and two old populations 'Francofonte' and 'Santa Tecla') were evaluated regarding anthocyanin and non-anthocyanin phenolic contents using ultrahigh performance liquid chromatography (UHPLC)-Orbitrap-mass spectrometry (MS). Moreover, total antioxidant activity (TAA) was evaluated using a 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) assay. Twenty-three phenolic compounds were identified. Cyanidin-3,5-O-diglucoside and pelargonidin-3,5-O-diglucoside were the most representative anthocyanins in all genotypes; the Santa Tecla population had the highest content of these anthocyanins, 97.64 mg L-1 and 40.29 mg L-1 respectively. In the Francofonte population, ferulic acid hexoside was the most abundant compound (391.18 mg L-1 ). TAA values ranged between 221.5 and 36.73 µmol Trolox equivalents/100 mL of juice. A high TAA value was recorded for the Santa Tecla pomegranate population. The UHPLC-Orbitrap-MS approach was employed for the first time to identify the phenolic compound profiling in five pomegranate genotypes. TAA was analysed u...Continue Reading

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Citations

Mar 22, 2019·Natural Product Research·Vita Di Stefano, Maria Grazia Melilli
Apr 22, 2020·Critical Reviews in Food Science and Nutrition·Maryam PirzadehMohammad S Mubarak
Aug 30, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Antonella Di SottoSilvia Di Giacomo
Sep 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wamidh H TalibIntisar Hadi Al-Yasari
Dec 31, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Zihao ChangLanzhen Zhang
Apr 4, 2021·International Journal of Molecular Sciences·Álvaro Fernández-OchoaAntonio Segura-Carretero
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Valeria GuarrasiSilvia Vilasi

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