Antioxidant Activity/Capacity Measurement. 3. Reactive Oxygen and Nitrogen Species (ROS/RNS) Scavenging Assays, Oxidative Stress Biomarkers, and Chromatographic/Chemometric Assays

Journal of Agricultural and Food Chemistry
Reşat ApakEsra Capanoglu


There are many studies in which the antioxidant potential of different foods have been analyzed. However, there are still conflicting results and lack of information as a result of unstandardized assay techniques and differences between the principles of the methods applied. The measurement of antioxidant activity, especially in the case of mixtures, multifunctional or complex multiphase systems, cannot be evaluated satisfactorily using a simple antioxidant test due to the many variables influencing the results. In the literature, there are many antioxidant assays that are used to measure the total antioxidant activity/capacity of food materials. In this review, reactive oxygen and nitrogen species (ROS/RNS) scavenging assays are evaluated with respect to their mechanism, advantages, disadvantages, and potential use in food systems. On the other hand, in vivo antioxidant activity (AOA) assays including oxidative stress biomarkers and cellular-based assays are covered within the scope of this review. Finally, chromatographic and chemometric assays are reviewed, focusing on their benefits especially with respect to their time saving, cost-effective, and sensitive nature.


Feb 1, 1992·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·O I AruomaJ Löligers
Nov 6, 1991·Biochemical Pharmacology·G R Haenen, A Bast
Jan 1, 1990·Methods in Enzymology·B Halliwell, J M Gutteridge
Apr 1, 1990·Trends in Biochemical Sciences·J M Gutteridge, B Halliwell
Jun 1, 1989·Journal of Biochemical and Biophysical Methods·J T Corbett
Nov 23, 1988·Biochimica Et Biophysica Acta·A J Kettle, C C Winterbourn
Jan 1, 1986·Journal of Free Radicals in Biology & Medicine·E K Miller, I Fridovich
Mar 1, 1980·Archives of Biochemistry and Biophysics·E FinkelsteinE J Rauckman
May 1, 1982·Archives of Biochemistry and Biophysics·G M RosenE J Rauckman
Oct 1, 1982·Analytical Biochemistry·L C GreenS R Tannenbaum
Aug 13, 1980·Biochimica Et Biophysica Acta·C D AllredH R Hill
Sep 25, 1995·Biochemical and Biophysical Research Communications·S A van AckerA Bast
Aug 1, 1995·Free Radical Biology & Medicine·S von KruedenerE F Elstner
Jul 15, 1993·European Journal of Biochemistry·H Sies
Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L J IgnarroR E Byrns
Jan 1, 1995·Critical Reviews in Food Science and Nutrition·B HalliwellO I Aruoma
Jun 15, 1994·Biochemical and Biophysical Research Communications·L MarcocciL Packer
Feb 1, 1994·Free Radical Biology & Medicine·N W KooyJ S Beckman
Oct 1, 1993·Free Radical Biology & Medicine·K Faulkner, I Fridovich
Mar 1, 1993·Free Radical Biology & Medicine·G CaoR G Cutler
Jan 1, 1993·Critical Reviews in Toxicology·J P Kehrer
Mar 15, 1996·Archives of Biochemistry and Biophysics·L J YanL Packer
Jan 1, 1997·Free Radical Biology & Medicine·G CaoR L Prior
Jan 1, 1997·Free Radical Biology & Medicine·H BussC C Winterbourn
Jul 25, 1997·The Journal of Biological Chemistry·I Fridovich
Oct 29, 1999·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·D Anderson, B J Phillips
Dec 10, 1999·Journal of Agricultural and Food Chemistry·M P KähkönenM Heinonen
Jan 21, 2000·Free Radical Biology & Medicine·M J DaviesR T Dean
Jan 21, 2000·Free Radical Biology & Medicine·R L Prior, G Cao
Apr 1, 2000·The Journal of Nutritional Biochemistry·K SuetsunaH Ochi

❮ Previous
Next ❯


Sep 30, 2016·Journal of Agricultural and Food Chemistry·Mengmeng ZhangHui Wu
Apr 14, 2017·Journal of Agricultural and Food Chemistry·Amrita ChaudharyJyotirmoy Chatterjee
Dec 27, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rui GengYixi Xie
Jun 26, 2020·Frontiers in Physiology·Hannah ChewAlfred N Fonteh
Mar 30, 2017·Critical Reviews in Food Science and Nutrition·Zipora Tietel, Segula Masaphy
Dec 10, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Najlaa BassalatNidal Jaradat
Feb 23, 2021·Critical Reviews in Food Science and Nutrition·Xuan ChenZeyuan Deng
May 17, 2021·Food Research International·Yang YiHong-Xun Wang
Apr 17, 2018·The Journal of Organic Chemistry·Yohei OkadaHidehiro Kamiya
Mar 28, 2019·Organic Letters·Kaii NakayamaYohei Okada
Jul 2, 2019·Journal of Agricultural and Food Chemistry·Reşat Apak
Oct 27, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xinyue GuoJilin Tang

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.