Aug 21, 2013

Antioxidant and genoprotective effects of spent coffee extracts in human cells

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
Jimena BravoConcepción Cid


Spent coffee has been shown as a good source of hydrophilic antioxidant compounds. The ability of two spent coffee extracts rich in caffeoylquinic acids, mainly dicaffeoylquinic acids, and caffeine (Arabica filter and Robusta espresso) to protect against oxidation and DNA damage in human cells (HeLa) was evaluated at short (2 h) and long (24 h) exposure times. Cell viability (MTT) was not affected by spent coffee extracts (>80%) up to 1000 μg/mL after 2 h. Both spent coffee extracts significantly reduced the increase of ROS level and DNA strand breaks (29-73% protection by comet assay) induced by H₂O₂. Pretreatment of cells with robusta spent coffee extract also decreased Ro photosensitizer-induced oxidative DNA damage after 24 h exposure. The higher effectiveness of Robusta spent coffee extract, with less caffeoylquinic acids and melanoidins, might be due to other antioxidant compounds, such as caffeine and other Maillard reaction products. This work evidences the potential antioxidant and genoprotective properties of spent coffee in human cells.

Mentioned in this Paper

DRUG Screen Quant Caffeine
Petroleum distillate
Hydrogen Peroxide
Dimethyl Sulfoxide
Lymphocytes as Percentage of Blood Leukocytes (Lab Test)
Inosine Dialdehyde
Antioxidant Effect

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