Antioxidant and hepatoprotective effects of artichoke extracts and constituents in cultured rat hepatocytes

Toxicology in Vitro : an International Journal Published in Association with BIBRA
R Gebhardt, M Fausel

Abstract

Primary cultures of rat hepatocytes exposed to tert-butyl hydroperoxide (t-BHP) were used for characterizing the antioxidative and hepatoprotective potential of aqueous artichoke extracts (AK.) and some selected constituents. Addition of t-BHP to the culture medium resulted in enhanced lipid peroxidation measured as production of malondialdehyde (MDA) and enhanced cytotoxicity detected by LDH leakage. Aqueous artichoke extracts added prior or simultaneously with t-BHP reduced both phenomena with EC(50) values of about 95 and 12 mug artichoke powder/ml, respectively. Furthermore, AE prevented the loss of intracellular GSH caused by t-BHP. Several polyphenolic and flavonoid constituents of AE were found to reduce MDA production. EC(50) values were 8.1, 12.5, 15.2, 28 mug/ml for caffeic acid, chlorogenic acid, cynarin and cynarosid, respectively. A similar ranking for AE and constituents was found when using the chemiluminescent xanthine oxidase assay for determination of the antioxidative potency, but EC(50) values were consistently lower. Concerning the hepatoprotective effect, that is prevention of LDH leakage, all constituents were almost equipotent and EC(50) values were lower than for MDA production. These results suggest (i...Continue Reading

References

Jul 1, 1976·Analytical Biochemistry·J E Brehe, H B Burch
Aug 1, 1995·Toxicology in Vitro : an International Journal Published in Association with BIBRA·X PonsodaJ V Castell
Jul 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·K MertensA Vercruysse
Jul 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S CoeckeV Rogiers

❮ Previous
Next ❯

Citations

Mar 15, 2006·Analytical and Bioanalytical Chemistry·Katrin SchützAndreas Schieber
Oct 5, 2007·Biological Trace Element Research·Ebru GurelCihan Demirci
Oct 4, 2011·Indian Journal of Clinical Biochemistry : IJCB·R Jayanthi, P Subash
Jun 16, 2005·Journal of Ethnopharmacology·Akram JamshidzadehHossein Niknahad
Oct 1, 2011·Asian Pacific Journal of Tropical Biomedicine·Sundaram Ravikumar, Murugesan Gnanadesigan
Aug 16, 2012·Journal of Medicinal Food·Laura Vicedo JacociunasRafael Rodrigues Dihl
Sep 29, 2009·Neurochemistry International·Sanja Pavlica, Rolf Gebhardt
Mar 25, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Sanja PavlicaRolf Gebhardt
May 6, 2015·The Journal of Eukaryotic Microbiology·Juergen Voigt, Johannes Woestemeyer
Aug 30, 2008·Fundamental & Clinical Pharmacology·Krishnamoorthy Karthikesan, Leelavinothan Pari
Jul 20, 2007·Fundamental & Clinical Pharmacology·L Pari, K Karthikesan
Feb 8, 2005·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·S M WittemerM Veit
Apr 2, 2002·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·G GlässerR Gebhardt
Feb 24, 2001·Free Radical Research·F Pérez-GarcíaS Cañigueral
Nov 22, 2005·Free Radical Research·Sanja Pavlica, Rolf Gebhardt
May 1, 2009·Environmental Toxicology and Pharmacology·Rolf Gebhardt
Nov 4, 2016·Food & Function·Imma PaganoLuca Rastrelli
Apr 2, 2014·Genetics and Molecular Biology·Laura Vicedo JacociunasHeloísa Helena Rodrigues de Andrade
May 19, 2018·Electrophoresis·Imma PaganoLuca Rastrelli
Oct 23, 2018·Phytotherapy Research : PTR·Shabnam SalekzamaniKhatereh Rezazadeh
Aug 30, 2012·Andrologia·J ThilagavathiR Dada
Jul 28, 2019·Nutrients·Nerea Jiménez-MorenoCarmen Ancín-Azpilicueta
Nov 5, 1998·Free Radical Research·J E Brown, C A Rice-Evans
May 26, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Maryem Ben SalemKhaled Mounir Zeghal
Aug 14, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ana Miklavčič Višnjevec, Matthew Schwarzkopf
Aug 9, 2002·Journal of Agricultural and Food Chemistry·Kotamballi N Chidambara MurthyRavendra P Singh
Aug 5, 2004·Journal of Agricultural and Food Chemistry·Simona GrandeFrancesco Visioli
Oct 3, 2002·Journal of Agricultural and Food Chemistry·Kotamballi N Chidambara MurthyGuddadarangavvanahally K Jayaprakasha

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.