PMID: 22338549Feb 18, 2012Paper

Antioxidant, antiglycation and inhibitory potential of Saraca ashoka flowers against the enzymes linked to type 2 diabetes and LDL oxidation

European Review for Medical and Pharmacological Sciences
A PrathapanK G Raghu

Abstract

The present study investigated the antioxidant, antiglycation and inhibitory potential of flavonoid fraction of Saraca ashoka flowers (SAF) against alpha-glucosidase and alpha-amylase (the enzymes linked to type 2 diabetes) and LDL oxidation. Antioxidant capacity of SAF was evaluated by estimating total antioxidant activity (TAA) and its protective effects against the oxidative stress induced by H2O2 on C2C12 cells. Cytotoxicity by MTT assay and markers of oxidative stress: reduced glutathione (GSH), malondialdehyde (MDA) and reactive oxygen species (ROS) were measured. Pre-treatment of C2C12 cells with SAF prevented the increased formation of MDA and depletion of GSH induced by H2O2. The increased ROS generation induced by H2O2 was also reduced by a pretreatment with SAF. Significant inhibitory potential against alpha-glucosidase and alpha-amylase enzymes revealed the therapeutic potential of SAF as an antihyperglycemic agent. SAF also demonstrated potent antiglycation property and inhibited LDL oxidation under in vitro conditions. The overall results demonstrate that SAF can be used as an ideal natural remedy for preventing oxidative stress and other complications associated with diabetes.

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.