Antioxidant defenses in the peripheral cell layers of legume root nodules

Plant Physiology
D A DaltonJ M Chatfield

Abstract

Ascorbate peroxidase (AP) is a key enzyme that scavenges potentially harmful H2O2 and thus prevents oxidative damage in plants, especially in N2-fixing legume root nodules. The present study demonstrates that the nodule endodermis of alfalfa (Medicago sativa) root nodules contains elevated levels of AP protein, as well as the corresponding mRNA transcript and substrate (ascorbate). Enhanced AP protein levels were also found in cells immediately peripheral to the infected region of soybean (Glycine max), pea (Pisum sativum), clover (Trifolium pratense), and common bean (Phaseolus vulgaris) nodules. Regeneration of ascorbate was achieved by (homo)glutathione and associated enzymes of the ascorbate-glutathione pathway, which were present at high levels. The presence of high levels of antioxidants suggests that respiratory consumption of O2 in the endodermis or nodule parenchyma may be an essential component of the O2-diffusion barrier that regulates the entry of O2 into the central region of nodules and ensures optimal functioning of nitrogenase.

References

Oct 1, 1993·Plant Physiology·M Chatfield, D A Dalton
Apr 1, 1996·Archives of Biochemistry and Biophysics·D A DaltonJ M Chatfield
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·R Sánchez-FernándezM J May
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D A DaltonH J Evans
Oct 1, 1992·The New Phytologist·Ann M Hirsch

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Citations

Jan 5, 2001·Phytochemistry·J B FrueaufM J Kieliszewski
Oct 26, 2002·Trends in Plant Science·Axel Mithöfer
Jun 25, 2013·Journal of Agricultural and Food Chemistry·K Judith WebbAna L Winters
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Jun 13, 2006·Plant Physiology·Manuel A MatamorosManuel Becana
May 13, 2011·Plant Physiology·Alejandro Tovar-MéndezManuel Becana
Apr 15, 2014·Plant Physiology and Biochemistry : PPB·Mohamed Lazali, Jean Jacques Drevon
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Jul 6, 2011·The New Phytologist·Sarra El MsehliPierre Frendo
Oct 15, 2009·Molecular Plant·Christine ChangPierre Frendo
Jun 5, 2013·Plant Signaling & Behavior·Adnane BargazJean-Jacques Drevon
Jan 8, 2015·Frontiers in Plant Science·K Judith WebbAna L Winters
Sep 1, 2004·FEMS Microbiology Ecology·David A DaltonChristina Kennedy
Jul 6, 2005·Journal of Experimental Botany·Cristina Ortega-VillasanteLuis E Hernández
Aug 3, 2005·Journal of Experimental Botany·Beata A WoluckaDirk Inzé
Mar 3, 2004·Microbiology·Frédérique Barloy-HublerFrancis Galibert
May 6, 2020·The New Phytologist·Viviana EscuderoManuel González-Guerrero
Sep 12, 2018·International Journal of Molecular Sciences·Md Mosfeq-Ul HasanXuede Wang
Feb 23, 2020·Frontiers in Plant Science·Viviana EscuderoManuel González-Guerrero

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