Antioxidant effect of resveratrol in single red blood cells measured by thermal fluctuation spectroscopy

Archives of Biochemistry and Biophysics
María José GallardoBenjamín Sepúlveda

Abstract

The human red blood cell (RBC) membrane has significant elastic capabilities which can be described measuring typical membrane edge fluctuations and mechanical properties by optical techniques. The RBC elastic properties can be affected by changes in the surrounding media. In an attempt to elucidate the molecular mechanisms of the interaction of resveratrol with the red cell membrane and of its antioxidant capacity the changes in mechanical properties of the RBC membrane were analyzed. These studies were carried out through measurements of RBC membrane fluctuations in the presence of the oxidant agent HClO using thermal fluctuation spectroscopy (TFS). The observed results showed that the elastic capabilities of RBC changed with low concentration of hypochlorous acid but without morphological changes. However, in the presence of resveratrol the deformation and decrease of elastic capabilities induced by HClO on RBC decreased. These in vitro results demonstrated the protective effect of RV against the detrimental effects triggered by HClO upon human erythrocytes.

References

Nov 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M P Sheetz, S J Singer
Jan 31, 1997·Biochimica Et Biophysica Acta·J Y Chen, W H Huestis
Feb 19, 2000·Life Sciences·L Frémont
Feb 22, 2001·Free Radical Biology & Medicine·I B ZavodnikM Bryszewska
Feb 21, 2002·Journal of Agricultural and Food Chemistry·Christelle PrivatFrançoise Nepveu
Apr 5, 2002·Medicinal Research Reviews·J Middleton Boon, Bradley D Smith
Dec 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Gerald Lim H WRanjan Mukhopadhyay
Sep 19, 2003·Biochemical and Biophysical Research Communications·Stephen S LeonardXianglin Shi
Nov 26, 2004·Journal of Agricultural and Food Chemistry·Francesco CarusoMiriam Rossi
Jul 3, 2007·Optics Letters·Michael GöglerJosef Alfons Käs
Sep 1, 2009·Proceedings of the National Academy of Sciences of the United States of America·Timo BetzCécile Sykes
Jan 9, 2013·Journal of Medicinal Chemistry·Stalin SelvarajUma Maheswari Krishnan
Oct 1, 2014·Biochimica Et Biophysica Acta·M SuwalskyM J Gallardo
Dec 31, 2015·Physical Biology·Fernanda GárateRoberto Bernal
Jul 28, 2018·Frontiers in Cellular Neuroscience·Fernanda GárateRoberto Bernal

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.