Antioxidant effects of equol on bovine aortic endothelial cells

Biochemical and Biophysical Research Communications
Jae-Eun ChungMee-Ran Kim

Abstract

This study is to examine the effects of equol on the H(2)O(2)-induced death of bovine aortic endothelial cells (bAECs) and the mechanism of its protective effects. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide] assay showed that in the control group, cell survival rate decreased significantly, each in proportion to the duration of the H(2)O(2) stimulation (P<0.05), but, in the equol-pretreated group, such decrease was not statistically significant. After Hoechst 33342 staining, in the equol-pretreated group the number of cells with apoptotic morphology decreased significantly. Equol pretreatment effectively inhibited the H(2)O(2)-induced cell death by the reduction of intracellular ROS production (P<0.05). Incubation of bAECs with equol increased the expression of phospho-p38 MAPK and Bcl-2 after the H(2)O(2) exposure compared with their expression without the equol pretreatment. Furthermore, SB203580 inhibited phospho-p38 MAPK expression and increased apoptotic cell death. This study proves equol has a significant antioxidant effect on the bAECs that were exposed to H(2)O(2).

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Citations

Nov 16, 2011·In Vitro Cellular & Developmental Biology. Animal·Xiao-Jing WeiRu-Qian Zhao
Mar 24, 2012·Animal : an International Journal of Animal Bioscience·X J WeiR Q Zhao
Jun 4, 2010·The Journal of Nutrition·Kenneth D R Setchell, Carlo Clerici
May 6, 2010·Journal of Zhejiang University. Science. B·Abdul Hamid HafizahAbdullah Mahdy Zaleha
Apr 18, 2020·American Journal of Hypertension·Takayuki MatsumotoTsuneo Kobayashi
Jun 20, 2017·Journal of the American College of Nutrition·Michael K RegerJianjun Zhang
Jun 4, 2019·Biological & Pharmaceutical Bulletin·Takayuki MatsumotoTsuneo Kobayashi

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