PMID: 8967516May 1, 1996Paper

Antioxidant enzyme expression in rat lungs during hyperoxia

The American Journal of Physiology
Y S HoJ D Crapo

Abstract

To understand the molecular mechanisms that upregulate the activities of pulmonary antioxidant enzymes in adult rats during exposure to 85% oxygen, the relative contents of corresponding mRNA in normal and hyperoxic lungs were determined. Hyperoxic exposure drastically induced the expression of lung manganese-containing superoxide dismutase (MnSOD) mRNA. Maximal induction of MnSOD mRNA occurred at days 3 and 5 of exposure to hyperoxia, reaching a 600 and a 340% increase over the levels of air-exposed rats, respectively. In addition, hyperoxia induced lung mRNA for glucose-6-phosphate dehydrogenase, glutathione peroxidase, glyceraldehyde-3-phosphate dehydrogenase, alpha-tubulin, and gamma-actin to different extends at various days of exposure. Hyperoxia had little or no effect on the levels of mRNA for copper/zinc-containing superoxide dismutase (CuZnSOD), catalase, heat shock protein (HSP70), and creatine kinase. Nuclear run-on experiments showed that the transcriptional rate of the MnSOD gene is enhanced in hyperoxic rat lungs by approximately 400% at day 3 of exposure compared with that of controls. The specific activities of CuZnSOD and MnSOD in these lung samples per unit of lung protein or DNA were also determined. The act...Continue Reading

Citations

Feb 9, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·G YangP A Dennery
Jun 11, 2003·American Journal of Respiratory and Critical Care Medicine·Vuokko L Kinnula, James D Crapo
May 13, 2006·American Journal of Respiratory Cell and Molecular Biology·Arnaud ChambellanSerpil C Erzurum
Apr 14, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Daniel D Lee, Margaret A Schwarz
Feb 9, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·S R RiddleC W White
Oct 4, 2000·Physiological Reviews·V Hampl, J Herget
May 8, 2000·Journal of Applied Physiology·D O Wilson, P Johnson
Mar 11, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ivan T DemchenkoBarry W Allen
Jul 10, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Han-Suk KimAron B Fisher
May 10, 2011·Journal of Applied Physiology·Zhuohui GanSaid H Audi
Jul 5, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Said H AudiMarilyn P Merker
Jan 10, 2006·American Journal of Respiratory Cell and Molecular Biology·Yan WangAron B Fisher
Sep 14, 2006·Journal of Applied Physiology·A Mark Evans, Constancio Gonzalez
Aug 11, 2000·The American Journal of Medicine·B M Babior

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