Antioxidant enzymes, hematology and histology of spleen in Nile tilapia fed supplemented diet with natural extracts challenged with Aeromonas hydrophila

Fish & Shellfish Immunology
Geovana DottaMaurício Laterça Martins

Abstract

This study investigated the effects of dietary supplementation with the extrats of propolis and Aloe barbadensis (aloe) on the antioxydant enzime activity, hematology and histology of the spleen of Nile tilapia challenged with Aeromonas hydrophila. Seventy two juvenile Nile tilapia were divided in four treatments and three replicates and fed extract mixture for 15 days: fish fed supplemented diet with 1% of the mixture of extracts of propolis and aloe (1:1) injected with phosphate-buffered saline (PBS); fish fed suplemented diet with 1% of the mixture of extracts of propolis and aloe (1:1) injected with the A. hydrophila, fish fed supplemented diet with the mixture of propolis extracts and aloe, injected with PBS and injected with A. hydrophila. The influence of the supplementation of propolis and Aloe extracts on the immunomodulation in tilapias was observed by the evaluation of the survival of the animals after challenge with A. hydrophila. Non-supplemented fish had a 44.5% survival rate and those supplemented with 1% of the mixture of extracts showed 55.6% survival 7 days after challenge. The supplemented animals also showed a significant increase in the number of lymphocytes in the evaluation of the blood parameters and, co...Continue Reading

Citations

Jan 6, 2021·Antioxidants·Francisca FélixElsa Cabrita
Jan 1, 2021·Journal of Applied Microbiology·G Bandeira Junior, B Baldisserotto
Feb 21, 2021·Journal of Environmental Management·Catarina ViegasMargarida Gonçalves

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