Antioxidant kinetics in lung lavage fluid following exposure of humans to nitrogen dioxide

American Journal of Respiratory and Critical Care Medicine
F J KellyT Sandstrom

Abstract

To determine if nitrogen dioxide (NO2), a gaseous free radical, modifies the protective antioxidant pool present in respiratory tract lining fluids, a random, double-blind study utilizing flexible fiberoptic bronchoscopy with bronchial and bronchoalveolar lavage was performed. Healthy, nonsmoking, asymptomatic subjects were exposed to filtered air and 2 ppm NO2 for 4 h on separate occasions. To examine the kinetics of the NO2-induced antioxidant reactions, 44 subjects were randomly assigned to one of three groups. Bronchoscopy was performed 1.5 h (group 1), 6 h (group 2) or 24 h (group 3) after each exposure. Reduced glutathione (GSH), uric acid, and ascorbic acid concentrations were determined in both bronchial and bronchoalveolar lavage fluid fractions. In addition, bronchoalveolar lavage fluid was screened for malondialdehyde as a marker of lipid peroxidation. Exposure to NO2 resulted in a rapid (1.5 h) loss of uric acid from the bronchial region, however by 6 h after exposure it had increased significantly above control uric acid concentration in this region. At 24 h after exposure, uric acid concentration had returned to the control level. A similar response of uric acid to NO2 was seen in the bronchoalveolar region. Ascor...Continue Reading

Citations

Feb 12, 2004·Archives of Biochemistry and Biophysics·Ian S MudwayFrank J Kelly
Jan 21, 2004·Free Radical Biology & Medicine·Reinhold SchmidtAndreas Günther
May 11, 2000·Molecular Aspects of Medicine·I S Mudway, F J Kelly
Oct 29, 1999·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·F J Kelly
Aug 17, 1999·Pathology, Research and Practice·P J Barth, B Müller
Mar 3, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M T Krishna, S T Holgate
Feb 26, 2000·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A Blomberg
Oct 7, 2000·Respirology : Official Journal of the Asian Pacific Society of Respirology·R OzarasA Cetinkaya
Sep 23, 2003·Critical Care Medicine·Russell P BowlerBrian J Day
Oct 31, 2002·Equine Veterinary Journal. Supplement·C M DeatonR C Schroter
Mar 18, 2000·Thorax·S O ShaheenP G Burney
Aug 1, 1997·American Journal of Respiratory and Critical Care Medicine·A BlombergT Sandström
Feb 2, 1999·American Journal of Respiratory and Critical Care Medicine·A BlombergT Sandström
Jun 14, 2000·American Journal of Respiratory and Critical Care Medicine·R A JörresH Magnussen
Oct 13, 2000·American Journal of Respiratory and Critical Care Medicine·N E AvissarJ N Finkelstein
Oct 28, 1998·Environmental Health Perspectives·C E CrossB Halliwell
Sep 15, 2011·Clinical Chemistry and Laboratory Medicine : CCLM·Sibel DorukZehra Seyfikli
Mar 18, 2015·The Science of the Total Environment·Tamás SzigetiGyula Záray
Aug 21, 2014·Oxidative Medicine and Cellular Longevity·Borut Poljšak, Rok Fink
Dec 17, 2015·Inhalation Toxicology·Gary E HatchUrmila P Kodavanti
May 23, 2009·Inhalation Toxicology·Annelie F BehndigIan S Mudway
Jan 6, 2010·Inhalation Toxicology·Jeremy P LangrishAnders Blomberg
Jul 6, 2000·Inhalation Toxicology·M J WiesterD L Costa
Dec 3, 2015·Environmental Research·David E DambyKenneth Donaldson
Jul 1, 2008·Environmental Toxicology and Pharmacology·Shufan QiAalt Bast
Aug 13, 2003·Journal of Clinical Pharmacy and Therapeutics·S L NuttallM J Kendall
Jul 13, 1999·Archives of Oral Biology·B ZappacostaB Giardina
Oct 10, 2006·International Journal of Immunopathology and Pharmacology·G ServiddioE Altomare
Mar 26, 2013·The Korean Journal of Internal Medicine·Min-Sung LeeAn-Soo Jang
Feb 22, 2003·Pediatric Research·Bettina C SchockMadeleine Ennis
Nov 2, 2017·Canadian Respiratory Journal : Journal of the Canadian Thoracic Society·Kambiz MasoumiHamidreza Aghababaeian
Feb 10, 1999·The American Journal of Physiology·A van der VlietS Louie

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