Antioxidant properties of recombinant allophycocyanin expressed in Escherichia coli

Journal of Photochemistry and Photobiology. B, Biology
Baosheng GeYuhong Ren

Abstract

Allophycocyanin (APC) is the main core component of phycobilisome found in blue-green algae. The apo-allophycocyanin and its subunits were expressed in Escherichia coli and their antioxidant properties were evaluated using deoxyribose assay. The result showed that both recombinant allophycocyanin fused with maltose binding protein (MBP) tag and 6 x His-tag and their alpha or beta subunits can scavenge hydroxyl radicals successfully, and the separated alpha or beta subunits had a higher inhibition effect on hydroxyl radicals than that when they combined together. The scavenging effects increased with the increasing concentration. These results clearly suggested that apo-allophycocyanin is involved in the antioxidant and radical scavenging activity of phycocyanin, and the antioxidant activity may be partially responsible to the anti-tumor effect of the recombinant allophycocyanin.

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Citations

Jun 20, 2008·Applied Microbiology and Biotechnology·Niels T Eriksen
Sep 12, 2013·Marine Drugs·Parisa HeydarizadehMartine Bertrand
Nov 17, 2009·Mutation Research·Katia G IvanovaRayna N Boteva
Mar 10, 2009·Journal of Bioscience and Bioengineering·Baosheng GeSong Qin
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Jul 17, 2010·Cardiovascular Therapeutics·Ruitang Deng, Te-Jin Chow
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Apr 3, 2008·Journal of Separation Science·Haixiang SunHuanlin Chen
Jun 9, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xian-Jun WuPing-Ping Li
Nov 1, 2016·Comprehensive Reviews in Food Science and Food Safety·Imen Hamed
Apr 27, 2021·Journal of Food Science and Technology·A ChandralekhaK S M S Raghavarao
Apr 29, 2021·Clinical and Experimental Pharmacology & Physiology·Sara MohitiMehrangiz Ebrahimi Mamaghani

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