Antioxidant response of cucumber (Cucumis sativus) exposed to nano copper pesticide: Quantitative determination via LC-MS/MS

Food Chemistry
Yuxiong HuangArturo A Keller

Abstract

Targeted metabolomics aims to provide a new approach to investigate metabolites and gather both qualitative and quantitative information. We describe a protocol for extraction and analysis of plant metabolites, specifically 13 secondary metabolites (antioxidants) using liquid chromatography coupled to triple quadrupole mass spectrometry (LC-MS/MS), with high linearity (R2 > 0.99) and reproducibility (0.23-6.23 R%) with low limits of detection (>0.001 ng/mL) and quantification (>0.2 ng/mL). The protocol was applied to study the antioxidant response of cucumber plants exposed to nanocopper pesticide. Dose-dependent changes in antioxidant concentrations were found, and 10 antioxidants were significantly consumed to scavenge reactive oxygen species, protecting plants from damage. Levels of three antioxidants were up-regulated, as a response to the depletion of the other antioxidants, signaling activation of the defense system. We demonstrated that the reported LC-MS/MS method provides a quantitative analysis of antioxidants in plant tissues, for example to investigate interactions between plants and nanomaterials.

Citations

Aug 16, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Raghavendhar R Kotha, Devanand L Luthria
Feb 6, 2020·Scientific Reports·Starlin Péguy Engozogho AnrisBertrand Charrier
Jun 7, 2019·Plants·Fabián Pérez-LabradaAntonio Juárez-Maldonado
Apr 17, 2020·The Science of the Total Environment·Yi WangJorge L Gardea-Torresdey
May 28, 2021·Environmental Science & Technology·Xiangning HuangArturo A Keller
Nov 28, 2021·Environmental Science and Pollution Research International·Shraddha Pravin Shahane, Arun Kumar

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