Antioxidant supplementation during in vitro culture improves mitochondrial function and development of embryos from aged female mice

Reproduction, Fertility, and Development
Elena SilvaRebecca L Krisher

Abstract

Maternal aging results in reduced oocyte and blastocyst quality, thought to be due, in part, to mitochondrial dysfunction and accumulation of reactive oxygen species. To reduce oxidative stress, the antioxidants α-lipoic acid (ALA; 10µM), α-tocopherol (250µM), hypotaurine (1mM) and N-acetylcysteine (NAC; 1mM), and sirtuin (100ngmL(-1)) were added to embryo culture medium (AntiOX) and compared with a control (CON) without antioxidants to assess blastocyst development after in vitro maturation and fertilisation of oocytes from aged B6D2F1 female mice (13.5 months). Development to the blastocyst stage increased in the AntiOX compared with CON group (87.6% vs 72.7%, respectively; P<0.01), in addition to higher mitochondrial membrane potential and ATP levels in the AntiOX group. Expression of genes associated with oxidative stress (PI3K, FOXO3A and GLRX2) was upregulated in the CON compared with AntiOX group. In addition to AntiOX, a medium containing only NAC and ALA (rAntiOX) was used to culture embryos from young CF1 females (6-8 weeks). More blastocysts developed in the rAntiOX compared with CON group (64.1% vs 43.3%, respectively; P<0.01), although AntiOX (48.0% blastocysts) did not result in improved development in young mice....Continue Reading

References

Sep 1, 1996·Molecular Reproduction and Development·L Janny, Y J Menezo
Aug 1, 1997·Fertility and Sterility·A S Lim, M F Tsakok
Sep 25, 2001·American Journal of Obstetrics and Gynecology·J Klein, M V Sauer
Jun 19, 2004·Journal of Assisted Reproduction and Genetics·Ana Karina BartmannRui Alberto Ferriani
Sep 27, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Kimberly A PreisDavid K Gardner
Jan 16, 2007·Current Topics in Developmental Biology·Rémi DumollardJohn Carroll
Jan 24, 2007·Maternal and Child Health Journal·Suzanne ToughChristine Newburn-Cook
Oct 31, 2007·Zygote : the Biology of Gametes and Early Embryos·C SuzukiM Takahashi
Jun 17, 2009·Seminars in Cell & Developmental Biology·R DumollardK Swann
Jun 23, 2009·Journal of Assisted Reproduction and Genetics·S M HosseiniMohammad H Nasr-Esfahani
Jul 13, 2011·The Journal of Cell Biology·Toren Finkel
Jul 21, 2011·Molecular Reproduction and Development·Ye YuanRebecca L Krisher
Dec 6, 2011·Cell Death and Differentiation·E C FerberA Schulze
Dec 27, 2011·Fertility and Sterility·Matthew RabinowitzZachary Demko
Jan 11, 2012·Journal of Assisted Reproduction and Genetics·Ali TalebiIsaac Karimi
Feb 24, 2012·Human Reproduction·Jinmiao LiuLin Liu
Mar 1, 2012·Molecular Reproduction and Development·Elena SilvaRebecca L Krisher
Feb 26, 2013·Free Radical Biology & Medicine·Stefan I Liochev
Jun 12, 2013·Cell Metabolism·Jonathan L Tilly, David A Sinclair
Jun 15, 2013·Obstetrics and Gynecology International·Scott Chappel
Dec 18, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Liang ZhangQiang Wang
Jan 25, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Kouji KomatsuFumitaka Kikkawa
Jun 26, 2014·Human Reproduction·Giovanna Di EmidioCarla Tatone
Aug 26, 2014·Epigenetics : Official Journal of the DNA Methylation Society·Azizollah Bakhtari, Pablo J Ross
Aug 26, 2014·The International Journal of Biochemistry & Cell Biology·Moncef BenkhalifaHenri Copin

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Citations

Sep 8, 2015·Journal of Assisted Reproduction and Genetics·Jolene FredricksonDean E Morbeck
Apr 21, 2016·Journal of Assisted Reproduction and Genetics·Tian XiaChune Chao
May 12, 2016·Human Reproduction·Thi T TruongDavid K Gardner
May 8, 2020·Systems Biology in Reproductive Medicine·Manuel BelliGuido Macchiarelli
May 5, 2017·Scientific Reports·Li-Feng LiangWei-Hua Wang
Oct 9, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rolando PasquarielloMargherita Maranesi
Jan 11, 2021·Reproduction in Domestic Animals = Zuchthygiene·Yuanyuan HeFusheng Quan
Dec 3, 2020·Antioxidants·Cristina Rodríguez-Varela, Elena Labarta
Mar 14, 2021·Reproductive Sciences·Zhixin Jiang, Huan Shen

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Methods Mentioned

BETA
Assay
confocal microscopy
PCR

Software Mentioned

Olympus
NCSS Cruncher
Primer3
Fluoview FV10
REST

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