Antioxidant treatment protects against matrix metalloproteinase activation and cardiomyocyte injury during acute pulmonary thromboembolism.

Naunyn-Schmiedeberg's Archives of Pharmacology
Ozélia Sousa-SantosJose E Tanus-Santos

Abstract

Increased reactive oxygen species (ROS) promote matrix metalloproteinase (MMP) activities and may underlie cardiomyocyte injury and the degradation of cardiac troponin I (cTI) during acute pulmonary thromboembolism (APT). We examined whether pretreatment or therapy with tempol (a ROS scavenger) prevents MMP activation and cardiomyocyte injury of APT. Anesthetized sheep received tempol infusion (1.0 mg kg(-1) min(-1), i.v.) or saline starting 30 min before or 30 min after APT (autologous blood clots). Control animals received saline. Hemodynamic measurements were performed. MMPs were studied in the right ventricle (RV) by gelatin zymography, fluorimetric activity assay, and in situ zymography. The ROS levels were determined in the RV and cTI were measured in serum samples. APT increased the pulmonary arterial pressure and pulmonary vascular resistance by 146 and 164%, respectively. Pretreatment or therapy with tempol attenuated these increases. While APT increased RV + dP/dt (max), tempol infusions had no effects. APT increased RV MMP-9 (but not MMP-2) levels. In line with these findings, APT increased RV MMP activities, and this finding was confirmed by in situ zymography. APT increased the RV ROS levels and tempol infusion, be...Continue Reading

References

Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H E Van Wart, H Birkedal-Hansen
Jan 24, 2003·Critical Reviews in Biochemistry and Molecular Biology·Philippe E Van den SteenGhislain Opdenakker
Aug 9, 2003·Current Opinion in Pulmonary Medicine·Kenneth T Horlander, Kenneth V Leeper
Nov 5, 2003·Circulation·Nils Kucher, Samuel Z Goldhaber
Dec 6, 2003·British Journal of Pharmacology·Britt ElmedalUlf Simonsen
Dec 24, 2004·European Heart Journal·Manoj M LaluRichard Schulz
Jun 25, 2005·Anesthesia and Analgesia·Carlos A Dias-JuniorJose E Tanus-Santos
May 3, 2006·International Journal of Cardiology·Geisa M FortunaJose E Tanus-Santos
Jul 4, 2006·Journal of Molecular and Cellular Cardiology·John A WattsJeffrey A Kline
Sep 23, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Kai KappertArne Ostman
Oct 13, 2006·The American Journal of the Medical Sciences·Shailja V Parikh, James A de Lemos
Nov 30, 2006·Annual Review of Pharmacology and Toxicology·Richard Schulz
Dec 13, 2006·Free Radical Biology & Medicine·Carlos A C Dias-JuniorJose E Tanus-Santos
Jun 27, 2007·British Journal of Pharmacology·A K ChowR Schulz
Dec 1, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·Juliana A UzuelliJose E Tanus-Santos
Apr 22, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Vincent G DeMarcoKevin C Dellsperger
Sep 24, 2008·International Journal of Experimental Pathology·John Albert WattsJohn Zagorski
Oct 7, 2008·Basic & Clinical Pharmacology & Toxicology·Carlos A Dias-JuniorJose E Tanus-Santos
Mar 3, 2009·Free Radical Biology & Medicine·Michele M CastroJose E Tanus-Santos
Mar 10, 2009·Pharmacology & Therapeutics·Ulf SimonsenNiels Henrik Buus
Aug 7, 2009·Cardiovascular Research·Arulmozhi D KandasamyRichard Schulz
Dec 9, 2009·Matrix Biology : Journal of the International Society for Matrix Biology·Michele M CastroRaquel Fernanda Gerlach
Aug 10, 2010·Naunyn-Schmiedeberg's Archives of Pharmacology·Marcelo F MontenegroJose E Tanus-Santos
Sep 2, 2010·Nitric Oxide : Biology and Chemistry·Carlos A Dias-JuniorJose E Tanus-Santos
Feb 1, 2011·Journal of Natural Products·Fabricio O SoutoWaldiceu A Verri

❮ Previous
Next ❯

Citations

Apr 10, 2013·Cardiovascular Drugs and Therapy·Stefany B A CauJose E Tanus-Santos
Aug 21, 2013·Nature Reviews. Cardiology·Jose E Tanus-Santos
Aug 24, 2012·Basic & Clinical Pharmacology & Toxicology·Karina C FerrazJose E Tanus-Santos
Jun 16, 2014·The Journal of Surgical Research·Job C TharappelJ Scott Roth
Sep 24, 2013·Biochimica Et Biophysica Acta·Paras Kumar MishraSuresh C Tyagi
May 10, 2019·Pharmaceutical Biology·Zhi-Yao WuYong-Jun Tang
Nov 26, 2019·The Journal of Surgical Research·Vashisht MadabhushiJohn Scott Roth

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.