Antioxidative and antiinflammatory activities of asiatic acid, glycyrrhizic acid, and oleanolic acid in human bronchial epithelial cells

Journal of Agricultural and Food Chemistry
Shih-ming Tsao, Mei-chin Yin

Abstract

Protective effects of triterpenic acids, asiatic acid (AA), glycyrrhizic acid (GA), or oleanolic acid (OA), for two human bronchial epithelial cells, 16HBE and BEAS-2B cells, against hydrogen peroxide (H2O2) induced injury were examined. Cells were pretreated by triterpenic acid at 4 or 8 μmol/L and followed by H2O2 treatment. Results showed that H2O2 significantly upregulated both Bax and cleaved caspase-3 expression, and also downregulated Bcl-2 expression in test cells. AA at these doses retained Bcl-2 expression, but GA and OA only at 8 μmol/L reserved Bcl-2 expression. Test triterpenic acids lowered cleaved caspase-3 expression dose-dependently. H2O2 treatment lowered Na(+)-K(+)-ATPase activity and mitochondrial membrane potential in cells. Triterpenic acid pretreatments significantly maintained mitochondrial membrane potential and Na(+)-K(+)-ATPase activity. H2O2 enhanced reactive oxygen species, interleukin-6, tumor necrosis factor-α, and prostaglandin E2 levels in test cells. Three triterpenic acid treatments dose-dependently reversed these changes. H2O2 promoted the protein expression of p47(phox), gp91(phox), cyclooxygenase-2 (COX-2), mitogen-activated protein kinase, and nuclear factor-κB (NF-κB). AA, GA, or OA pretr...Continue Reading

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Citations

Apr 29, 2016·BMC Complementary and Alternative Medicine·Putcharawipa ManeesaiPoungrat Pakdeechote
Jul 6, 2016·Journal of Ethnopharmacology·Lara F AzevedoTati Ishikawa
Aug 6, 2016·International Immunopharmacology·Jae-Won LeeHyun-Jun Lee
Sep 23, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiang HuangYisong Qian
Sep 11, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Yufeng RenJihong Zhang
Mar 11, 2017·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Dong-Geun KangJin Sung Park
Jan 1, 2020·Journal of Asian Natural Products Research·Yan-Ling SongYan-Qiu Meng
Feb 28, 2020·Journal of Asian Natural Products Research·Yan-Qiu MengXiao-Xiao Li
Feb 23, 2018·Experimental and Therapeutic Medicine·Xi-Li MiaoDa-Peng Wan
Jan 12, 2019·Cell Communication and Signaling : CCS·Hongming LvHaihua Feng
Feb 20, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Trevor Tapiwa NyakudyaKennedy Honey Erlwanger
Jun 5, 2020·CNS & Neurological Disorders Drug Targets·Saeed SamarghandianTahereh Farkhondeh

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