Antioxidative and protective properties of extracts from leaves of the artichoke (Cynara scolymus L.) against hydroperoxide-induced oxidative stress in cultured rat hepatocytes

Toxicology and Applied Pharmacology
R Gebhardt

Abstract

Primary rat hepatocyte cultures exposed to tert-butylhydroperoxide (t-BHP) or cumene hydroperoxide were used to assess the antioxidative and protective potential of water-soluble extracts of artichoke leaves. Both hydroperoxides stimulated the production of malondialdehyde (MDA), particularly when the cells were pretreated with diethylmaleate (DEM) in order to diminish the level of cellular glutathione (GSH). Addition of artichoke extracts did not affect basal MDA production, but prevented the hydroperoxide-induced increase of MDA formation in a concentration-dependent manner when presented simultaneously or prior to the peroxides. The effective concentrations (down to 0.001 mg/ml) were well below the cytotoxic levels of the extracts which started above 1 mg/ml. The protective potential assessed by the LDH leakage assay and the MTT assay closely paralleled the reduction in MDA production and largely prevented hepatocyte necrosis induced by the hydroperoxides. The artichoke extracts did not affect the cellular level of glutathione (GSH), but diminished the loss of total GSH and the cellular leakage of GSSG resulting from exposure to t-BHP. Chlorogenic acid and cynarin accounted for only part of the antioxidative principle of the...Continue Reading

Citations

Apr 23, 2009·Natural Product Research·Xian-Feng ZhuRamond Lo
Jan 5, 2012·BMC Genomics·Davide ScaglioneSergio Lanteri
Jan 26, 2012·BMC Genomics·Domenico De PaolaGabriella Sonnante
Feb 2, 2010·Biological & Pharmaceutical Bulletin·Kazuo IshidaMikio Ito
Nov 7, 2014·Case Reports in Hepatology·Hüseyin KurtErdoğan Bülbül
Feb 24, 2001·Free Radical Research·F Pérez-GarcíaS Cañigueral
Sep 29, 2009·Neurochemistry International·Sanja Pavlica, Rolf Gebhardt
May 12, 2009·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·R HuberR Lüdtke
Mar 25, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Sanja PavlicaRolf Gebhardt
May 1, 2009·Environmental Toxicology and Pharmacology·Rolf Gebhardt
Mar 10, 2016·Pharmaceutical Biology·Dianelena Eugenio-PérezJosé Pedraza-Chaverri
Sep 12, 2006·Journal of Ethnopharmacology·Michele Luciane DickelMara Rejane Ritter
Aug 28, 2015·Plant Foods for Human Nutrition·Maryem Ben SalemKhaled Mounir Zeghal
Apr 2, 2002·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·G GlässerR Gebhardt
Jan 1, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Ela Nur Simsek, Tuna Uysal
Nov 22, 2005·Free Radical Research·Sanja Pavlica, Rolf Gebhardt
Jul 5, 2005·Expert Opinion on Investigational Drugs·J WuM A Zern
Sep 23, 2014·Pharmaceutical Biology·Engy M El Morsy, Rehab Kamel
Sep 13, 2012·Biochimica Et Biophysica Acta·Vicente SaezPier Giorgio Righetti
Oct 16, 2013·Water Research·C ConidiE Garcia-Castello
Oct 30, 2016·Journal of Agricultural and Food Chemistry·Youssef RouphaelLuigi Lucini
Jun 25, 2005·The American Journal of Chinese Medicine·Tai-Hyun KangYoun-Chul Kim
Nov 8, 2001·Free Radical Research·A R RechnerC A Rice-Evans
Aug 5, 2009·Biological Trace Element Research·Canan KüçükgerginMüjdat Uysal
Nov 4, 2016·Food & Function·Imma PaganoLuca Rastrelli

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