Antioxidative effects of glycoprotein isolated from Solanum nigrum L

Journal of Medicinal Food
Kyung-Sun Heo, Kye-Taek Lim

Abstract

Glycoprotein from Solanum nigrum L. (SNL glycoprotein) was isolated and tested for antioxidative effects on oxygen free radicals using a 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The free radical scavenging activities of the SNL glycoprotein are optimal in acidic pH and up to 60 degrees C. However, it has minimal activities in the presence of EDTA, although such activities are not dependent on M(2+) ions (Ca(2+), Mn(2+), and Mg(2+)) in the presence of EDTA. Interestingly, when SNL glycoprotein was treated with deactivation agents (pronase E and NaIO(4)), the DPPH radical scavenging activity was decreased compared with the SNL glycoprotein treatment alone. The antioxidative effects of SNL glycoprotein on superoxide anion and hydroxyl radical under optimal conditions revealed that SNL glycoprotein has remarkable scavenging effects on both radicals, but exhibited slightly higher scavenging effects on superoxide anion generated by the enzymatic hypoxanthine/xanthine oxidase system than on hydroxyl radicals generated by the Fenton reaction. However, SNL glycoprotein was more effective against hydroxyl radials in cell cultures (NIH/3T3). Consequently, 20 microg/mL SNL glycoprotein has a scavenging ability against superoxide anion c...Continue Reading

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Citations

Sep 22, 2010·World Journal of Gastroenterology : WJG·Roja RahimiMohammad Abdollahi
Nov 2, 2006·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Zainul Amiruddin ZakariaMohd Roslan Sulaiman
Jun 10, 2010·Journal of Agricultural and Food Chemistry·Giuseppe MennellaRoberto Lo Scalzo

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