Antioxidative enzymes activities and accumulation of steroids in hairy roots of Trigonella.

Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology
Somayeh KohsariMasoud Mirmasoumi

Abstract

Steroidal sapogenins and phytosterols are a group of secondary metabolites which are very considerable in the pharmaceutical industry. Fenugreek (Trigonella foenum-graecum L.) is the good source of these compounds. In recent decades, there is a great interest to production of these compounds by cultivation of transformed roots. In present study, hairy roots induction in two Trigonella species (T. foenum-graeceum, T. monantha) with three strains of Agrobacterium rhizogenes (15,834, A4 and wt) was investigated. Transgenic status of roots was confirmed by PCR using rolB specific primers. Virulence of these strains was examined on explants of leaf, leaf cotyledone and hypocotyle in both species. The best strain was wt for hairy root induction in hypocotyle and leaf explants of T. foenum-graeceum and T. monantha. Significant quantitative differences were showed between shoot, root and hairy roots in both species. Protein content in root and hairy root of both species was significantly lower in comparison with shoot. Superoxide dismutase (SOD) and peroxidase (POX) activities in hairy roots of both species were higher as compared to other organs. The hairy roots of both species showed an ability to synthesize steroidal sapogenins. The...Continue Reading

References

Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·J J DoyleA H Brown
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS
Sep 21, 2004·Annual Review of Plant Biology·Klaus Apel, Heribert Hirt
Sep 1, 1942·Journal of Bacteriology·H J Conn
Feb 1, 1977·Plant Physiology·C N Giannopolitis, S K Ries
Jan 1, 1991·Plant Physiology·F B Abeles, C L Biles
Jun 19, 2007·Phytochemistry·Fabricio Medina-BolivarMaureen C Dolan
Nov 7, 2008·Molecular Plant-microbe Interactions : MPMI·Victor P BulgakovYuri N Zhuravlev
Jun 1, 1997·Plant Cell Reports·A MerkliI Kapetanidis
Jun 7, 2019·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Ahmed M M GabrMarian Brestic

❮ Previous
Next ❯

Citations

Nov 24, 2020·Applied Microbiology and Biotechnology·Saikat Gantait, Eashan Mukherjee

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
transgenic

Software Mentioned

SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved