Antiphase dual-color correlation in a reactant-product pair imparts ultrasensitivity in reaction-linked double-photoswitching fluorescence imaging

Journal of the American Chemical Society
Wei WanAlexander D Q Li

Abstract

A pair of reversible photochemical reactions correlates their reactant and product specifically, and such a correlation uniquely distinguishes their correlated signal from others that are not linked by this reversible reaction. Here a nanoparticle-shielded fluorophore is photodriven to undergo structural dynamics, alternating between a green-fluorescence state and a red-fluorescence state. As time elapses, the fluorophore can be in either state but not both at the same time. Thus, the red fluorescence is maximized while the green fluorescence is minimized and vice versa. Such an antiphase dual-color (AD) corelationship between the red and green fluorescence maxima as well as between their minima can be exploited to greatly improve the signal-to-noise ratio, thus enhancing the ultimate detection limit. Potential benefits of this correlation include elimination of all interferences originating from single-color dyes and signal amplification of AD photoswitching molecules by orders of magnitude.

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Citations

Feb 26, 2016·ACS Applied Materials & Interfaces·Lingyang ZhangXuanyong Liu
Oct 8, 2015·Nanoscale·Yuanlin ZhangAlexander D Q Li
Feb 3, 2016·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Jérôme QuérardLudovic Jullien
Feb 9, 2016·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Rindia M PutriNathalie Katsonis
Nov 26, 2015·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·T UdayabhaskararaoRafal Klajn
Aug 16, 2017·Chemistry, an Asian Journal·Yasuko OsakadaTetsuro Majima
Aug 13, 2021·Physical Chemistry Chemical Physics : PCCP·Junzhao HeGuohua Jiang
Sep 18, 2018·Chemical Reviews·Honglin Li, Joshua C Vaughan
Jan 2, 2019·The Journal of Physical Chemistry Letters·Haitao ZhouSuotang Jia

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