Antiphospholipid antibodies limit trophoblast migration by reducing IL-6 production and STAT3 activity

American Journal of Reproductive Immunology : AJRI
Melissa J MullaVikki M Abrahams

Abstract

Women with antiphospholipid antibodies (aPL) are at risk of recurrent miscarriage and pre-eclampsia. aPL target the placenta by binding to beta(2)-glycoprotein I (beta(2) GPI) expressed by the trophoblast. The objective of this study was to evaluate if and how aPL affect first trimester trophoblast migration. First trimester trophoblast cells were treated with anti-beta(2) GPI monoclonal antibodies. Migration was determined using a two-chamber assay. Interleukin (IL)-6 production was evaluated by RT-PCR and enzyme-linked immunosorbent assay, and signal transducer and activator of transcription 3 (STAT3) activation was assessed by western blot. Trophoblast cells constitutively secreted IL-6 in a time-dependent manner and this directly correlated with STAT3 phosphorylation. In the presence of anti-beta(2) GPI Abs, trophoblast IL-6 mRNA levels and secretion was downregulated in a Toll-like receptor 4/MyD88-independent manner and this correlated with a reduction in phosphorylated STAT3 levels. In addition, the anti-beta(2) GPI Abs reduced the migratory potential of trophoblast. Heparin was able to reverse aPL-dependent inhibition of trophoblast IL-6 secretion and migration. This study demonstrates that aPL limit trophoblast cell mi...Continue Reading

References

Mar 20, 1998·American Journal of Reproductive Immunology : AJRI·N S RoteA K Ng
Aug 15, 1998·American Journal of Obstetrics and Gynecology·M S MagidM D Lockshin
Apr 8, 1999·Clinical and Experimental Immunology·L W ChamleyP M Johnson
Oct 12, 2000·Baillière's Best Practice & Research. Clinical Obstetrics & Gynaecology·L Regan, R Rai
Oct 10, 2001·Fertility and Sterility·L W ChamleyP M Johnson
Apr 16, 2002·Arthritis and Rheumatism·Ricard CerveraUNKNOWN Euro-Phospholipid Project Group
Oct 19, 2002·Journal of Reproductive Immunology·L W Chamley
Jun 12, 2003·Obstetrics and Gynecology·D Ware Branch, Munther A Khamashta
Dec 14, 2004·American Journal of Obstetrics and Gynecology·Patrick BoseBerthold Huppertz
Jan 27, 2005·American Journal of Obstetrics and Gynecology·Patrick BoseBerthold Huppertz
Aug 30, 2005·Annals of the New York Academy of Sciences·Guido Valesini, Cristiano Alessandri
Mar 25, 2006·Molecular Human Reproduction·Frank A HillsJan J Brosens
Dec 13, 2006·American Journal of Reproductive Immunology : AJRI·Melissa J CostelloVikki M Abrahams
Feb 20, 2007·Lancet·David P D'CruzGraham R V Hughes
Feb 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Peter J Murray
Jul 6, 2007·Human Reproduction·R GanapathyB Thilaganathan
Sep 26, 2007·Annals of the New York Academy of Sciences·Nicoletta Di SimoneCaruso Alessandro
Oct 17, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Yoshihiko SawaShigemitsu Yoshida
Nov 30, 2007·Nihon Naika Gakkai zasshi. The Journal of the Japanese Society of Internal Medicine·Yoshiya Tanaka
Apr 22, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Vikki M AbrahamsGil Mor
Mar 6, 2009·American Journal of Reproductive Immunology : AJRI·Melissa J MullaVikki M Abrahams
Jul 21, 2009·American Journal of Reproductive Immunology : AJRI·Melissa J MullaVikki M Abrahams

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Citations

Oct 22, 2011·Obstetrics and Gynecology·Christina S HanVikki M Abrahams
Apr 1, 2011·International Journal of Clinical Rheumatology·Brock E HarperSilvia S Pierangeli
Aug 29, 2013·Clinical & Developmental Immunology·T MarchettiP de Moerloose
Jul 24, 2012·Journal of Reproductive Immunology·Jelmer R PrinsSarah A Robertson
Jul 28, 2012·American Journal of Reproductive Immunology : AJRI·Ae Ra HanJoanne Kwak-Kim
May 7, 2011·American Journal of Reproductive Immunology : AJRI·Tamara Y CarrollVikki M Abrahams
Mar 26, 2013·American Journal of Reproductive Immunology : AJRI·Joanne Kwak-KimAlice Gilman-Sachs
Jul 30, 2014·American Journal of Reproductive Immunology : AJRI·Stefan M GyslerVikki M Abrahams
Dec 12, 2013·American Journal of Reproductive Immunology : AJRI·Caroline R AlbertVikki M Abrahams
Mar 25, 2014·Journal of Thrombosis and Haemostasis : JTH·T MarchettiM Cohen
Sep 24, 2015·Birth Defects Research. Part C, Embryo Today : Reviews·Clifton O'neill BrockSarah Gloria Običan
Dec 4, 2014·American Journal of Reproductive Immunology : AJRI·Katie PoultonIan P Giles
Feb 3, 2015·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·Yukun LiuJianping Zhang
Mar 31, 2015·Clinical Hemorheology and Microcirculation·Georg-Friedrich von TempelhoffRoland Csorba
Mar 13, 2015·Current Rheumatology Reports·Rohan WillisA R Brasier
Feb 18, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Priyadarshini PanthamLawrence W Chamley
Aug 1, 2013·Reproductive Sciences·Marianna Pina RambaldiMichael J Paidas
Nov 15, 2017·American Journal of Reproductive Immunology : AJRI·Zola Chihombori QuaoVikki M Abrahams
Jan 18, 2018·Arthritis & Rheumatology·Melissa J MullaVikki M Abrahams
Sep 4, 2018·American Journal of Reproductive Immunology : AJRI·Kathleen R HeimVikki M Abrahams
Sep 9, 2018·American Journal of Reproductive Immunology : AJRI·Daisy Leon-MartinezVikki M Abrahams
Mar 5, 2019·Molecular Genetics & Genomic Medicine·Junhong CaiShan Bao
Jan 13, 2018·Nature Reviews. Disease Primers·Karen SchreiberBeverley J Hunt
Jan 21, 2020·Journal of Internal Medicine·E Svenungsson, A Antovic
Oct 18, 2014·Journal of Perinatal Medicine·Katarina JeremicJelena Jeremic
Dec 18, 2010·Expert Review of Clinical Immunology·Amolak S BansalMeen-Yau Thum
Jun 7, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mancy TongVikki M Abrahams
Nov 1, 2011·Auto- Immunity Highlights·Rohan Willis, Silvia S Pierangeli
Jan 4, 2021·Ecotoxicology and Environmental Safety·Lihao HuHui Chen
Nov 14, 2020·International Journal of Molecular Sciences·Roberta MisasiAntonella Capozzi
May 29, 2021·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Jamilya KhizroevaTamara Mashkova
Sep 3, 2021·Immunological Medicine·Kayoko KanekoAtsuko Murashima

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