Antiproliferative action of metformin in human lung cancer cell lines

Oncology Reports
Hironori AshinumaKoichiro Tatsumi

Abstract

The oral antidiabetic agent metformin has anticancer properties, probably via adenosine monophosphate-activated protein kinase activation. In the present study, growth inhibition was assessed by a clonogenic and by a cell survival assay, apoptosis induction was assessed by Hoechst staining and caspase activities and cell cycle alteration after exposure to metformin, and the interaction of metformin with cisplatin in vitro were elucidated in four human lung cancer cell lines representing squamous, adeno-, large cell and small cell carcinoma. Clonogenicity and cell proliferation were inhibited by metformin in all the cell lines examined. This inhibitory effect was not specific to cancer cells because it was also observed in a non-transformed human mesothelial cell line and in mouse fibroblast cell lines. Inhibition of clonogenicity was observed only when the cells were exposed to metformin for a long period, (10 days) and the surviving fraction, obtained after inhibiting proliferation by increasing the dose, reached a plateau at approximately 0.1-0.3, indicating the cytostatic characteristics of metformin. Metformin induced significant apoptosis only in the small cell carcinoma cell line. A tendency of cell cycle accumulation at ...Continue Reading

Citations

Mar 19, 2013·European Journal of Pharmacology·Christos V Rizos, Moses S Elisaf
Oct 30, 2012·Cardiovascular Diabetology·Aibek E Mirrakhimov
Oct 9, 2013·International Journal of Oncology·Satoru KitazonoKoichiro Tatsumi
Oct 13, 2015·Drug Discovery Today·Roberto WürthFederica Barbieri
May 20, 2015·The Veterinary Journal·K SaekiT Nakagawa
Feb 1, 2015·Oral Oncology·Daniela Fortunato RêgoEliete Neves Silva Guerra
Jul 20, 2016·Cancers·Kamal AhmedVladimir L Katanaev
Nov 1, 2017·Cancer Chemotherapy and Pharmacology·Daniela I Paiva-OliveiraCélia M F Gomes
Jul 22, 2019·Archives of Toxicology·Sabrina HeinzHeidrun Ellinger-Ziegelbauer
May 10, 2017·Cancers·Michael Yousef, Evangelia Tsiani

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