Antiproliferative activity and apoptosis induction by trijuganone C isolated from the root of Salvia miltiorrhiza Bunge (Danshen)

Phytotherapy Research : PTR
Takuhiro UtoYukihiro Shoyama

Abstract

In this study, we found that the hexane fraction of Danshen, the dried root of Salvia miltiorrhiza (Lamiaceae), exerted antiproliferative effects on human leukemia cells. Phytochemical investigation of the hexane fraction achieved the isolation of the tanshinone diterpenes: dihydrotanshinone I (1), trijuganone C (2), trijuganone B (3), cryptotanshinone (4), tanshinone IIA (5), and tanshinone I (6). Compound 2 showed significant antiproliferative activities against human leukemia cells HL-60, Jurkat, and U937. The antiproliferative activities of 2 against human cancer and normal cells indicated that 2 exhibited potent antiproliferative activities with IC50 values less than 10 μM against HL-60 and Jurkat cells as well as on the colon cancer cells DLD-1, COLO 205, and Caco-2. Compound 2 induced chromatin condensation, DNA fragmentation, activation of caspase-3, -8, and -9, and the cleavage of poly (ADP-ribose) polymerase (PARP) in HL-60 cells. Moreover, 2 activated Bid and Bax, leading to the loss of mitochondrial membrane potential, and 2 induced the cytochrome c release from mitochondria into cytosol. In contrast, Bcl-2 and Bcl-xL were unaffected by 2. These results suggest that 2 exerts antiproliferative effects via apoptosis i...Continue Reading

References

May 1, 1983·Chemical & Pharmaceutical Bulletin·M OnitsukaH B Maruyama
Jun 30, 2000·Current Opinion in Cell Biology·M Crompton
Feb 15, 2001·Cell Death and Differentiation·O Von AhsenD R Green
Mar 5, 2004·Biochimica Et Biophysica Acta·Martin R Sprick, Henning Walczak
Nov 18, 2005·Journal of Clinical Pharmacology·Limin ZhouMoses Sing Sum Chow
Mar 25, 2006·Phytotherapy Research : PTR·S HemaIswarya, Mukesh Doble
Feb 21, 2007·Journal of the Society for Integrative Oncology·Prachi GarodiaBharat B Aggarwal
Jun 15, 2007·Toxicologic Pathology·Susan Elmore
Apr 9, 2009·Chemistry & Biodiversity·Shu Jun ShengChun Guang Li
Jul 20, 2010·Bioorganic & Medicinal Chemistry Letters·Yue-Feng BiHong-Min Liu
Jul 19, 2011·Developmental Cell·Jean-Claude Martinou, Richard J Youle
Jul 7, 2012·Bioorganic & Medicinal Chemistry Letters·Yue-Feng BiHong-Min Liu
Feb 23, 2013·International Journal of Molecular Sciences·Takuhiro UtoYukihiro Shoyama
Mar 20, 2013·Biological Chemistry·Patrick HensleyNatasha Kyprianou
Apr 11, 2013·Pharmaceutical Biology·Zhiyu ZhangChun-Su Yuan
Aug 3, 2017·Phytotherapy Research : PTR·Nguyen Huu TungYukihiro Shoyama

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Citations

Aug 8, 2020·Journal of Asian Natural Products Research·Quan-Chang GuYa-Nan Yang
Jan 10, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Jie LiChanggang Sun
Mar 25, 2020·Journal of Natural Medicines·Shinya OkuboTakuhiro Uto
Nov 17, 2020·Drug Design, Development and Therapy·Rui GuoGuanwei Fan
Nov 26, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zhibei LiXiao Ma
Apr 26, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Deqian MengKai Wang

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